摘要
采用实验室试制的二价抗体接种5日龄SPF雏鸭,分别在注射抗体后24、48、72、96、120、144和168 h进行采血及分离血清。采用鸭胚中和试验,进行Ⅰ型和新型鸭肝炎病毒抗体效价的测定。结果表明,抗体注射24~72 h,血清中Ⅰ型和新型鸭肝炎病毒抗体效价基本稳定在峰值,从96 h开始缓慢下降但仍在保护效价（1：32）以上,至144 h抗体下降至保护效价以下,即抗体的被动保护期在5 d以内。
Egg yolk antibody preparing in labouratory inoculated 5-day-old SPF duck, collecting blood and separating serumafter the injection by 24, 48, 72, 96, 72, 96 and 168 hours, respectively. Testing the serum titer of I and new type of duck hepatitis antibody by duck embryo neutralization test.The results showed that serum antibody titer was basically stable at the peak between 24 to 72 h and slowed down from 96 h but still overtopped protecting titer （1：32）.The antibody titer dropped below to protecting titer from 144 h, so the passive protection of the antibody was 5 days.
出处
《广东畜牧兽医科技》
2016年第3期32-34,共3页
Guangdong Journal of Animal and Veterinary Science
关键词
卵黄抗体
血清效价
雏鸭
中和试验
egg yolk antibody,serum tilter, duckling,neutralization test
