摘要
目的:建立SD大鼠中等和高强度持续训练运动模型,探究不同训练负荷下,大鼠海马N-甲基-D-天冬氨酸受体(NMDARs)、突触后致密物质-95(PSD-95)和驱动蛋白家族蛋白17(KIF-17)表达及认知和情绪功能的变化。方法:实验建立SD大鼠跑台运动模型,分别进行中等和高强度持续训练4周,中等强度运动时采用递增负荷训练,高强度运动时采用高速训练,并设立正常对照组。测量运动后大鼠体质量减轻程度的变化,real-time PCR和Western blotting分别检测不同训练负荷对大鼠海马NMDA受体,PSD-95和KIF-17 m RNA和蛋白表达的影响。结果:相比对照组,中等强度持续运动精神状态明显更佳,高强度持续运动状态萎靡。中等强度持续训练组PSD-95、KIF-17和NMDA受体的m RNA和蛋白的表达显著上升(P<0.05),而高强度持续训练组m RNA和蛋白的表达则受到抑制(P<0.05)。结论:不同训练负荷对大鼠认知情绪及精神状态有较大影响,可以影响大鼠NMDA受体、PSD-95和KIF-17 m RNA和蛋白的表达。适量的运动训练不仅可以减轻缺氧对海马的损害,更可以有效地改善海马的功能。
Objective： To explore the changes of cognitive and emotional functions as well as the mRNA and protein ex- pressions of N-methyl-D-aspartate receptors （NMDARs）, postsynaptic density 95 （PSD-95） and kinesin family member 17 （KIF-17） in hippocampus for long-term moderate-intensity and high-intensity training models in rats. Method： The exercise model of SD rats were established by treadmill running of moderate or high intensity for 4 weeks. The rats in the moderate intensity group were given endurance training with incremental intensity, while the high-speed training in the high intensity group. And we also set the normal control group. The body weight of rats was measured before and after exercise in order to determine the reduction. Real-time PCR and Western blotting were used to detect the mRNA and protein expressions of NMDARs, PSD-95 and KIF-17 in hippocampus under different training loads. Result： Compared with those in the control group, the rats in the moderate-intensity group had better mental status, while a dispirited mental status in the high-intensity group. In the moderate-intensity group, the mRNA and protein expressions of PSD-95, KIF-17 and NMDARs were significantly increased （P〈0.05） with the sup-pressed expressions in the high-intensity group （P〈0.05）. Conclusion： Different training loads have great influences on the cognition, emotion and mental status of rats, and can affect the mRNA and protein expressions of NMDARs, PSD-95 and KIF-17. Appropriate training can not only alleviate the damage of hypoxia to hippocampus, but also effectively improve the function of hippocampus.
出处
《中国康复医学杂志》
CAS
CSCD
北大核心
2016年第7期746-751,共6页
Chinese Journal of Rehabilitation Medicine
基金
武警后勤学院重点项目(WKH2009Z02)
关键词
训练负荷
氧化应激
谷氨酸受体
认知
情绪
training load
oxidative stress
glutamate receptors
cognitive
emotions
