摘要
目的 探讨腹腔感染对腹膜淋巴孔的影响,以及选择性AT2血管紧张素Ⅱ受体拮抗剂PD123319对腹膜淋巴孔的调控机制.方法 采用实验研究方法.将40只大鼠采用随机数字表法,分为4组：正常对照组、假手术组、腹腔感染组、腹腔感染药物干预组,每组10只.腹腔感染组：按经典盲肠穿孔结扎实验建立腹腔感染模型.腹腔感染药物干预组：建立腹腔感染模型后,经腹腔注射PD123319水溶液（0.2 g/kg）.假手术组仅打开腹腔,翻动肠道后,即关闭腹腔.正常对照组、假手术组、腹腔感染组均经腹腔注射等量无菌生理盐水.2h后处死大鼠,采集腹膜组织标本.（1）采用扫描电镜观察腹膜淋巴孔变化,检测孔径及开放密度.（2）采用NO硝酸还原法,用全自动酶标仪检测腹膜组织NO浓度.（3）采用Westernblot法检测腹膜组织NO合酶内皮型一氧化氮合酶（eNOS）、Phospho-eNOS （P-eNOS）表达量.（4）采用流式细胞仪检测腹膜间皮细胞内钙离子浓度.正态分布的计量资料以-x±s表示,多组间比较采用方差分析,两两比较采用LSD检验.结果 （1）正常对照组、假手术组、腹腔感染组、腹腔感染药物干预组大鼠腹膜淋巴孔孔径分别为（2.3 ±0.4）μm、（2.5±0.5）μm、（4.7±0.5）μm、（3.8±0.5）μm,腹膜淋巴孔开放密度分别为（2.0±0.8）×10^8/m^2、（2.1±0.7） ×10^8/m^2、（6.2±1.3） ×10^8/m^2、（4.6±1.4）×10^8/m^2.4组大鼠腹膜淋巴孔孔径、腹膜淋巴孔开放密度比较,差异均有统计学意义（F =98.130,56.780,P＜0.05）.正常对照组大鼠腹膜淋巴孔孔径、腹膜淋巴孔开放密度与假手术组比较,差异均无统计学意义（t=1.281,0.514,P＞0.05）.腹腔感染组大鼠腹膜淋巴孔孔径、腹膜淋巴孔开放密度与正常对照组比较,差异均有统计学意义（t=11.586,8.573,P＜0.05）;腹腔感染组与腹腔感染药物干预组比较,差异均有统计学意义（t=3.854,3.098,P＜0.05）
Objective To investigate the effect of the peritoneal lymphatic stomata on intra-abdominal infection and the regulatory mechanism of angiotensin Ⅱ receptor specific inhibitor PD123319 on peritoneal lymphatic stomata.Methods The experimental study was adopted.Forty rats were divided into the control group,sham operation group,intra-abdominal infection group and intra-abdominal infection drug intervention group by the random number table,every group had 10 rats.The classic appendix perforation （CLP） intraabdominal infection model was established in the abdominal infection group.After establishing the model of abdominal infection,PD123319 solution was injected intraperitoneally immediately （0.2 g/kg） in the abdominal infection drug intervention group.Abdominal cavity of the rats in the sham operation group was opened,and then was shut after flipping the intestine.The rats in the control group,sham operation group and intra-abdominal infection group were treated with intraperitoneal injection of 1ml stroke-physiological saline solution.After 2 hours,the rats were sacrificed,and peritoneal tissue was taken for the following tests.（1） The aperture size and distribution density of peritoneal lymphatic stomata were observed by scanning electron microscope （SEM）.（2） The nitric oxide （NO） concentration in the peritoneal tissues was detected using nitric oxide nitric acid reduction method.（3） The expressions of endothelial nitric oxide synthase （eNOS） and Phospho-eNOS （P-eNOS） were detected by the Western blot.（4） The intracellular Ca2＋ concentration were detect by flow cytometry.Measurement data with normal distribution were presented as-x ± s.The comparison among groups was analyzed using the ANOVA and pairwise comparison was analyzed by the LSD test.Results （1） The aperture size and distribution density of the peritoneal lymphatic stomata in the control group,sham operation group,intra-abdominal infection group and intra-abdominal infection drug intervention group were res
出处
《中华消化外科杂志》
CAS
CSCD
北大核心
2016年第7期723-728,共6页
Chinese Journal of Digestive Surgery
基金
国家自然科学基金（81300280）
成都军区总医院院管课题（424121HE）
