摘要
目的探讨N-乙酰半胱氨酸(NAC)及氢化可的松对重症急性胰腺炎(SAP)的治疗保护作用及作用机制。方法72只SD大鼠随机分为4个组,假手术(SO)组、SAP组、NAC处理组及氢化可的松治疗组。采用胰胆管逆行注射5%牛磺胆酸钠诱导大鼠SAP模型;NAC处理组于模型制作前30min腹腔内注射NAC(200mg/kg)进行预处理;氢化可的松治疗组于SAP模型制作成功后10min给予舌下静脉注射氢化可的松琥珀酸钠(10mg/kg);假手术(SO)组开腹后仅翻动胰腺,即关腹。分别观察各组大鼠血浆淀粉酶、TNF-αI、L-6、肺湿/干比值、胰腺及肺组织病理以及组织髓过氧化物酶(MPO)的变化,采用RT-PCR法检测肺组织TNF-αmRNA的表达。结果SAP组血浆淀粉酶、TNF-α、IL-6、肺湿/干比值、肺组织TNF-αmRNA的表达、胰腺及肺组织MPO均较SO组明显升高,差异有统计学意义(P<0.05),NAC处理组及氢化可的松治疗组上述各检测指标均较SAP组显著降低,差异有统计学意义(P<0.05),NAC处理组胰腺及肺组织病理损伤明显减轻,氢化可的松治疗组肺组织病理损伤较SAP组减轻。结论NAC及氢化可的松可通过减少细胞因子TNF-αI、L-6的产生,对SAP具有一定的治疗保护作用。
Objective To investigate the therapeutic effect and mechanism of antioxidant and cortisone on severe acute pancreati- tis （SAP）. Methods Seventy-two SD rats were randomized into four groups：sham operation （SO）group,SAP group, N-acetylcysteine（NAC） -pretreated group and hydrocortisone-treated group. The model of SAP was induced by retrograde injection of 5% sodium taurocholate into the bile-pancreatic duct in SD rats, NAC-pretreated group was given 200mg/kg NAC intraperitoneally 30 min before inducing SAP, hydrocortisone -treated group was given 10mg/kg hydrocortisone 10 min after inducing SAP. The levels of amylase,TNF-α,IL-6, the wet /dry ratio of lung,and myeloperoxidase（MPO） in the pancreas and lung were measured. The his topathological changes of pancreatic and pulmonary tissue were evaluated. Intrapulmonary TNF-α mRNA was detected. Results The levels of amylase, TNF-α, IL-6, the MPO in the pancreas and lung, the wet /dry ratio of lung, and the expression of TNF-α mRNA in the lung were significantly increased in SAP group compared with those in SO group（P〈0.05）. Compared with SAP group, the indexes were significantly decreased in NAC-pretreated group and hydrocortisone -treated group（P〈0.05）, the severity of pancreas and lung injury also significantly decreased in NAC-pretreated group,the severity of lung injury was decreased in hydrocortisone -treated group. Conclusion Antioxidant（N-acetylcysteine） and cortisone（hydrocortisone） may have the therapeutic effect on SAP by decreasing the production of TNF-α and IL-6.
出处
《重庆医学》
CAS
CSCD
北大核心
2010年第2期137-139,共3页
Chongqing medicine
关键词
胰腺炎
乙酰半胱氨酸
激素
细胞因子
pancreatitis
N-Acetylcysteine
H ydrocortisone
cytokine
