摘要
目的观察己酮可可碱对重症急性胰腺炎(SAP)大鼠相关肺损伤的保护作用,并探讨其可能的发病机制。方法36只雌性SD大鼠随机分为假手术组、模型组、治疗组。采用5%牛磺胆酸钠逆行胰胆管注射的方法,复制大鼠SAP相关肺损伤模型。治疗组在模型制作前30min给予己酮可可碱。12h后分别对胰腺损伤、肺损伤程度进行病理评分,测定肺系数、胰腺湿重、血清淀粉酶,用ELISA法检测血清和支气管肺泡灌洗液中TNF-αI、L-6I、L-1β含量,用半定量RT-PCR法检测肺组织中NF-κB p65mRNA的表达。结果与模型组比较,治疗组胰腺及肺损伤程度明显减轻;其血清和支气管肺泡灌洗液中TNF-α、IL-6、IL-1β含量明显下降,肺组织中NF-κB p65 mRNA的表达亦明显减少。结论己酮可可碱对SAP相关急性肺损伤具有保护作用,其机制可能与抑制肺组织中NF-κB p65 mRNA的表达,从而抑制促炎细胞因子TNF-αI、L-6I、L-1β的产生相关。
Objective To investigate the protective effect and possible mechanism of pentoxifylline on severe acute pancreatitis associated lung injury in rats. Methods Thirty-six female Sprague Dawley （SD） rats were divided into sham operated group,model group and treatment group, 1mL/kg body weight of 5 % sodium taurocholate was retrogradely injected into the biliopancreatic duet of the rats to induce SAP associated lung injury. In treatment group, pentoxifylline was injected into caudal vein before 30min. Scores of pancreatic and pulmonary injury, plasma amylase, pancreatic wet weight and pulmonary coefficient were assessed. The value of TNF-α,IL-6,IL-1β in serum and bronchoalveolar lavage fluid（BALF） were detected by ELISA. The expression of NF-κB p65 mRNA in lung was detected by RT-PCR. Results To contrast with model group, the scores of pancreatic and pulmonary injury were lightened, the value of TNF-α,IL-6,IL-1β in serum and BALF was descented respectively, the expression of NF-κB p65 mRNA in lung was decreased in treatment group. Conslusion Pentoxifylline has the protective effect on severe acute panereatitis associated lung injury. The possible mechanism is correlated with that pentoxifylline inhibits the expression of NF-κB p65 mRNA in lung,and decreases the output of TNF-α,IL-6,IL-1β.
出处
《重庆医学》
CAS
CSCD
北大核心
2009年第24期3130-3131,3134,共3页
Chongqing medicine
