摘要
本研究利用PCR-SSCP技术结合DNA测序确定UCP2基因SNP位点,检测其在宁夏西门塔尔杂种牛群体中的多态性,并分析其与生长性状的关联性。结果表明,UCP2基因第1外显子存在3个SNPs位点,分别为T632C、A655G和A689C,结合PCR-SSCP结果分析后,分为AA、BB、AB 3种基因型。不同月龄体高、体重、胸围、体斜长,总体呈AA>AB>BB。AA基因型个体在不同月龄体高、胸围显著或极显著高于BB基因型(P<0.05;P<0.01),与AB基因型之间存在差异,但差异不显著(P>0.05)。可以确定,T632C位点发生的碱基突变对宁夏西门塔尔杂种牛的体重性状选育存在显著意义,AA基因型个体可作为后期选育方向。
To find genotype with fast growth rates in Ningxia hibrid Simmental cattle,the genetic variation of UCP 2 gene were detected by PCR-SSCP method and DNA sequencing,then analyzed UCP 2 gene polymorphism related with some growth traits.The results showed that there were three SNPs （T632C,A655G and A689C）in UCP2 gene exon 1,which divided into AA,BB and AB genotypes by PCR-SSCP method.Individuals with different genotypes in different months of age,body weight,body height,chest circumference and body length were AA〉AB〉BB.AA gen-otype in different age of body height and chest circumference were significantly different than BB genotype （P〈0.05;P〈0.01）,and were no significant difference with AB genotype （P〉0.05）. The results demonstrated that T632C locus had significant meaning for improving weight trait breeding of Ningxia hibrid Simmental cattle,AA genotype could be the breeding genotype.
出处
《中国畜牧兽医》
CAS
北大核心
2016年第7期1843-1849,共7页
China Animal Husbandry & Veterinary Medicine
基金
宁夏科技厅重大专项:优质高档肉牛新品系选育(2012ZDN0901)
