摘要
目的:探究电针对急性脊髓损伤大鼠成熟少突胶质细胞增殖分化的影响。方法:将24只SD雄性大鼠随机分为假手术组,模型组,电针组和药物组,每组各6只。采用重物坠落法制备急性脊髓损伤模型,术后均连续每天腹腔注射5-溴脱氧尿嘧啶核苷(Brd U,50mg/kg)作为细胞增殖标记,假手术组、模型组不予治疗,电针组予夹脊电针治疗(2/100Hz,20min),药物组予神经节苷脂(GM^(-1))治疗,连续治疗14d后处死老鼠,采用BBB评分评估大鼠脊髓损伤后的运动恢复情况,免疫荧光双标技术检测脊髓损伤组织Brd U表达,5-溴脱氧尿嘧啶核苷/髓鞘碱性蛋白(Brd U/MBP)共表达。结果:BBB评分显示,电针组BBB评分显著优于模型组(P<0.05)。免疫荧光双标显示,电针组Brd U表达和Brd U/MBP共表达与模型组相比显著升高(P<0.05)。结论:电针能提高脊髓损伤组织细胞Brd U/MBP阳性细胞共表达,促进成熟少突胶质细胞的增殖分化。
Objective： To explore the effects of electroacupuncture（EA） on the proliferation and differentiate of mature oligodendrocytes in spinal cord injury rats. Methods： A total of 24 SD rats were randomly and averagely divided into the shamoperated group, model group, EA group and drug group. Weight drop method was used to establish acute spinal cord injury（SCI） model, intraperitoneal injection 5-bromo-2-deoxyuridine（50mg/kg） after operation every day. Sham-operated group and model group without any treatment, electroacupuncture group received acupuncture on Jiaji points（EX-B2）（2/100 Hz, 20min） and rats in drug group were given treatment of ganglioside grease（GM^-1）. On day 14 after operation, those rats were sacrificed in each group. Using the BBB scale after spinal cord injury to assess motor function, double immunofluorescence detection were used to detect Brd U expression and Brd U/MBP co-expression in spinal cord injury tissue. Results： BBB score showed that EA group were significantly higher than those of sham operated group and model group（P〈0.05）. Double immunofluorescence showed that Brd U expression and Brd U/MBP co-expression in EA group were significantly increased than those of model group（P〈0.05）. Conclusion： EA can improve co-expression of Brd U/MBP-positive cells in spinal cord injury tissue which can promote mature oligodendrocytes proliferation and differentiation.
出处
《中华中医药杂志》
CAS
CSCD
北大核心
2016年第7期2771-2774,共4页
China Journal of Traditional Chinese Medicine and Pharmacy
基金
国家自然科学基金青年项目(No.81102644)
浙江省自然科学基金项目(No.Y2100533)~~
关键词
电针
免疫荧光双标
少突胶质细胞
髓鞘碱性蛋白
Electroacupuncture
Double immunofluorescence
Oligodendrocytes
Myelin basic protein
