摘要
本文以石墨烯(GR)和单壁碳纳米管(SWNTs)为修饰剂,研究其对金电极的增敏效果.并以邻氨基酚为功能单体,槲皮素为模板分子,于金电极表面通过电聚合制备对槲皮素具有特定选择性的分子印迹膜(MIP).采用循环伏安法(CV)和差分脉冲伏安法(DPV)研究了分子印迹膜的形成过程、结构、印迹效果和电化学性能.并用与槲皮素结构相似的化合物芦丁进行干扰实验,结果表明,该印迹膜对槲皮素有较好的选择性.优化了测定槲皮素的条件,绘制了槲皮素的工作曲线,该传感器对槲皮素浓度的线性响应范围3.00×10-7—1.92×10-5mol·L^(-1),线性方程为:I(μA)=^(-1)1.8505+2.6893lgc(mol·L^(-1)),(R=0.9935),检出限为1.00×10-7mol·L^(-1).5次重复测定结果的相对标准偏差为6.8%,放置10 d后,响应值为最初的92.5%,该传感器具有良好的重复性和稳定性.对黑茶样品中的槲皮素含量进行了测定,回收率在97.8%—104.0%之间,结果较为满意.
This paper studied the sensitizing effect of graphene（ GR） and single-walled carbon nanotubes（ SWNTs） which were employed for the surface modification of gold electrode. A molecularly imprinted membrane film selective to quercetin was prepared on the gold electrode by electropolymerization with o-aminophenol as functional monomers and quercetin as template molecule. The plymerization process,imprinting effect and the electrochemical properties of the molecularly imprinted film were investigated by the methods of cyclic voltammetry（ CV） and differential pulse voltammetry（ DPV）. Rutin which has similar structure with quercetin was used in the interference experiment. The result showed that the imprinted film had good selectivity to quercetin. Several parameters affecting the determination of quercetin were investigated andoptimized. In the range of 3. 00 × 10-7—1. 92 × 10-5mol·L^（-1）,the linear equation between peak current and quercetin concentration was I（ μA） =-11. 8505 ＋ 2. 6893lgc（ mol·L^（-1））（ R = 0.9935）,and the detection limit was 1.00×10-7mol·L^（-1）. This sensor have good reproducibility and stability,and it was used to determine the content of quercetin in black tea,a good recovery in the range of97.8%—104.0% was obtained.
出处
《环境化学》
CAS
CSCD
北大核心
2016年第6期1280-1286,共7页
Environmental Chemistry
基金
湖南省教育厅高校科研一般项目(15C0244)
国家自然科学基金(21545003)
湖南省自然科学基金(2015JJ2023)
湖南省高校科技创新团队支持计划(2014-207号)资助~~
