摘要
以槲皮素作为模板分子,邻氨基苯酚作为功能单体,在金电极表面通过电聚合法,制备了具有选择性识别槲皮素的分子印迹传感器。采用循环伏安法、差分脉冲伏安法研究了印迹膜的性能、结构和分子印迹效应。对功能单体与模板分子的配比、洗脱时间和印迹时间等实验参数进行了优化,并与其结构相似的化合物芦丁的选择性响应进行了比较,发现该传感器对槲皮素分子具有良好的选择性。槲皮素浓度在6.0×10^-6~1.0×10^-4mol/L范围内与峰电流呈线性关系,线性方程为：I（μA）=27.79＋9.48lgc（mol/L）（R=0.9939）,检出限为2.0×10^-6mol/L。用此传感器测定黑茶中槲皮素含量的结果较为满意。
Molecularly imprinted（ MIP） sensor,in which using quercetin as the template molecule,oaminophenol as functional monomer,on the surface of the gold electrode for detecting of quercetin were fabricated by electropolymerization method. Its performance,structure and molecular imprinting effect were investigated by cyclic voltammetry and differential pulse voltammetry. It exhibited good selectivity to quercetin with the interferences about rutin which has the similar structure as quercetin. Under the optimized conditions,the calibration curves are linear with quercetin concentration at the range of 6. 0 × 10^- 6~ 1. 0 × 10^- 4mol / L,and the linear equation was I（ μA） =27. 79 ＋ 9. 48lgc（ mol / L）（ R = 0. 9939） with detection limit of 2. 0 × 10^- 6mol / L. It has been successfully applied for the determination of quercetin in black tea.
出处
《化学通报》
CAS
CSCD
北大核心
2015年第10期918-922,共5页
Chemistry
基金
湖南省科技计划项目(2012GK3070)
湖南省应用基础研究基地开放基金项目(湘科计字2014HCKF03)
湖南省教育厅高校科研一般项目(15C0244)
湖南省高校科技创新团队支持计划资助项目[(2014)207]
益阳市指导性科技计划项目(2014JZ26)资助
关键词
槲皮素
分子印迹
差分脉冲伏安法
黑茶
Quercetin
Molecular imprinting
Differential pulse voltammetry
Black tea
