摘要
为了研究水环境中氯霉素(CAP)的快速检测方法,用单壁碳纳米管(SWCNTs)-纳米铜(CuNPs)复合物修饰玻碳电极(GCE),并结合分子印迹(MIP)技术,先后采用循环伏安法(CV)进行电极导电性测试,线性伏安法(LSV)对实验条件进行优化,差分脉冲伏安法(DPV)研究水溶液中CAP的电化学行为.研究结果表明,与GCE电极相比,修饰电极上CAP氧化还原电流信号明显增强,表明修饰电极对CAP具有较好的选择性,能加速电子传递.同时对洗脱液类型、缓冲溶液pH、扫描速度、富集时间及修饰物的负载量等实验条件进行了优化.在最佳条件下,发现CAP还原峰电流大小与其浓度在5—500 nmol·L^(-1)范围内呈现良好的线性关系,线性方程为I(μA)=0.9204C+41.285(R^2=0.9984),检出限为4.8 nmol·L^(-1)(S/N=3),且该修饰电极具有良好的重现性和稳定性.将该修饰电极应用于实际样品分析时,氯霉素眼药水的加标回收率为95.1%—102.5%,实际地表水样的加标回收率为96.5%—102.1%.
In order to study the rapid detection method of chloramphenicol (CAP) in water environment, single\walled carbon nanotubes (SWCNTs)-nano-copper (CuNPs) composites were used to modify glassy carbon electrodes (GCE) combined with molecular imprinting polymer (MIP) technology. Cyclic voltammetry (CV) was used to conduct electrode conductivity test. Linear scan voltammetry (LSV) was used to optimize the experimental conditions. Differential pulse voltammetry (DPV) was used to study the electrochemical behavior of CAP in aqueous solution. The results show that the CAP redox current signal of the modified electrode was significantly enhanced compared with the GCE electrode, which indicated that the modified electrode has strong selectivity to CAP and accelerated electron transport. At the same time, the experimental conditions were optimized, such as the eluent type, the pH value of the buffer solution, the scanning speed, the enrichment time and the loading of the modifier. Under the optimal conditions, it was found that the peak current of CAP reduction showed a good linear relationship with the concentration in the range of 5-500 nmol·L^-1. The linear equation is I(μA)=0.9204C + 41.285 (R2=0.9984). The limit is 4.8 nmol·L^-1 (S/N=3), and the modified electrode has the good reproducibility and stability. When the modified electrode was applied to the actual sample analysis, the spiked recovery rate of chloramphenicol eye drops was 95.1%-102.5%, and spike recovery of the actual surface water sample was 96.5%-102.1%.
作者
贾真真
解静芳
王振涛
姜洪进
任浦慧
JIA Zhenzhen;XIE Jingfang;WANG Zhentao;JIANG Hongjin;REN Puhui(College of Environment and Resource,Shanxi University,Taiyuan,030006,China)
出处
《环境化学》
CAS
CSCD
北大核心
2019年第3期513-521,共9页
Environmental Chemistry
基金
国家大气重污染成因与治理攻关项目(DQGG-05-11)
国家公益性行业(农业)科研专项(201103024)资助~~
关键词
氯霉素
分子印迹
碳纳米管-纳米铜
电化学检测
chloramphenicol
molecular imprinting
carbon nanotube-nano-copper
electrochemical detection
