摘要
目的为验证三叉神经节神经元上表达的水通道蛋白1（AQP1）和瞬时感受器电位离子通道4（TRPV4）可能参与痛觉传导提供形态学依据。方法应用免疫荧光染色结合FG逆行追踪标记技术比较观察口腔黏膜下注射多聚甲醛（PFA）实验模型组和对照组小鼠三叉神经节内AQP1和TRPV4的表达。结果与对照组比较，模型组三叉神经节神经元AQP1和TRPV4的表达无明显差异。结论存在于三叉神经节神经元上的AQP1和TRPV4可能未参与口腔伤害性刺激所引起的三叉神经痛觉的传递。
Objective To provide the morphological evidence for the hypothesis that aquaporin 1 （ AQP1 ） and transient receptor potential ion channel 4 （ TRPV4 ） in the trigeminal ganglion of mice might take participate in pain transduction. Methods The mice were given oral submucosal injection of paraformaldehyde, then immunofluorescence staining combined fluorogold （FG） retrograde tracing technique was performed to observe changes in AQP1 and TRPV4 expression in the trigeminal ganglion neurons that innervate the oral mucosa. Results Expression and distribution of AQP1 and TRPV4 in the trigeminal ganglion neurons that project to the oral mucosa was no significantly different between oral submucosal injection of paraformaldehyde-induced pain group and saline injection control group. Conclusion AOP1 and TRPV4 in the trigeminal ganglion may not take part in pain transduction.caused by the oral harmful stimulating.
出处
《浙江临床医学》
2016年第7期1194-1196,共3页
Zhejiang Clinical Medical Journal
基金
江苏省教育厅高校“青蓝工程”资助（苏教师[2014]23号）
