摘要
目的研究醛化白及多糖（DBsGM）/羟丙基壳聚糖（HPCS）/纳米珍珠层粉（NNP）复合支架材料对大鼠下颌骨缺损愈合过程的成骨促进作用,并观察该材料的降解吸收情况。方法取32只雄性Wistar大鼠,于每只大鼠双侧下颌磨牙区作箱型骨缺损,根据处死时间（2、4、6、8周）随机分成4组,每组8只,在4组中实验侧植入DBsGM/HPCS/NNP复合支架材料,对照侧不植入任何材料。切片经苏木精-伊红染色,组织学测量评价骨缺损修复效果。结果两组新生骨面积百分比随愈合时间延长逐渐增加（P〈0.05）;相同愈合时间的实验侧新生骨面积优于对照侧（P〈0.05）。植入材料随着时间逐渐降解,第6~8周,植入材料基本降解完全。结论 DBsGM/HPCS/NNP复合支架材料在大鼠下颌骨缺损中具有良好的促进成骨作用。
Objective To observe the osteogenesis and degradation of dialdehyde letilla striata glucomanna（ DBsGM） /hydroxypropyl chitosan（ HPCS） / nano-nacre powder（ NNP） composited scaffolds in rat alveolar bone defect regeneration. Methods A total of 32 male Wistar rats with bilateral mandibular molar area box defects were randomly divided into 4 groups according to the disposal time（ week 2,4,6,8）,with 8 animals in each group. The DBsGM / HPCS /NNP composited scaffolds were implanted in the experimental side,while nothing was implanted in the control side.The bone defect repair effect was evaluated with hematoxylin-eosin staining and histologic measurement. Results As time extended,the percentage of newbone area increased gradually,and there was significant difference between different time points（ P〈0. 05）. At the same time point,the experimental group was superior to the control group in gross observation and histological observation（ P〈0. 05）. The implanted materials gradually degraded with time. During week 6 to 8,the implanted materials completely degraded. Conclusion DBsGM / HPCS / NNP composited scaffolds can promote rat alveolar bone defect repair and regeneration.
出处
《山东大学学报（医学版）》
CAS
北大核心
2016年第6期7-11,30,共6页
Journal of Shandong University:Health Sciences
基金
山东省科技攻关计划(2014GGE27400)
关键词
白及胶
纳米珍珠层粉
组织工程
牙周组织再生
大鼠骨缺损
Bletilla striata glucomannan
Nano-nacre powder
Tissue engineering
Periodontal tissue regeneration
Periodontal defects in rats
