摘要
目的:改进少突胶质细胞体外培养方法。方法:取新生24 h内SD大鼠大脑皮质组织,采用机械性吸管吹打的方法获取细胞悬液,进行种植培养,恒温摇床结合无血清培养的方法纯化少突胶质细胞,Galc免疫细胞化学法鉴定少突胶质细胞。结果:纯化后的少突胶质细胞,胞体圆形或椭圆形,折光性强,突起少,经Galc免疫细胞化学染色,阳性细胞胞浆和突起呈棕黄色,少突胶质细胞纯度达95%。结论:采用此种方法培养出的少突胶质细胞纯化度高,且方便简易。
Objective： To improve the in vitro culture method for oligodendrocytes. Methods： Cells from cerebral cortex of 24 hours- old SD rats were disassociated by mechanical methods. Then,the cultured oligodendrocytes were purified using orbital shaker in constant temperature. The cultured oligodendrocytes were identified by immunocytochemical staining with anti- Galc antibody. Results： The purified oligodendrocytes showed round or oval cell body were and less cytoplasmic process. The cultured oligodendrocytes were identified by immunocytochemical staining with anti- Galc,which were stained brown in their cytoplasm and processes. The positive immunocytochemistry were counted more than 95 percents of all cells. Conclusion：The established method is simple,and the cultured oligodendrocytes is of high- grade purification.
出处
《黔南民族医专学报》
2016年第1期1-3,12,共4页
Journal of Qiannan Medical College for Nationalities
基金
贵州省科技厅社会发展攻关项目(黔科合SY字[2014]3024号)
贵阳市科技计划项目(筑科合同[20141001]21号)
