摘要
目的探讨胎牛血清对神经干细胞(neural stem cells,NSCs)分化结果的影响.方法通过无血清培养的方法,将胎鼠大脑皮质进行原代培养所获得的神经干细胞克隆传代纯化后,分别接种在加入DMEM/F12+B27和DMEM/F12+B27+5%FCS两种不同培养基的培养皿中诱导分化,9d后行免疫荧光细胞化学染色,用抗体β-Tubulin Ⅲ、GFAP、GalC鉴定分化细胞,并观察其形态特点.结果成功分离获得NSCs,并具有分化为神经元和胶质细胞的能力;在相同时间内含血清培养基中的细胞球分化快而充分,且分化结果不同.结论血清可促进NSCs分化并影响分化结果.
Objective To observe the effect of FCS on differentiation of neural stem cells. Methods The neural stem cells purified from primary cultured clones by serum-free media were placed into culture dishes with 2 different media neural basal, DMEM/F12＋B27 and DMEM/F12＋B27＋5% FCS respectively. After 9 days, immunofluorescence with antibody β-Tubulin Ⅲ , GFAP, GalC, the cells were identified and observed. Results NSCs were successfully isolated and cultured from cortex of fetal mice. These cells had the potential to differentiate into neurons and glia cells. Differentiation of NSCs was quick and sufficient in DMEM/F12＋B27＋5% FCS. Conclusion FCS can promote and influence the differentiation of neural stem cell.
出处
《昆明医学院学报》
2009年第5期33-36,共4页
Journal of Kunming Medical College
