摘要
目的:研究不同提取溶剂对独一味溶出成分的影响。方法:运用LC-MS/MS技术,针对独一味药材,结合色谱峰比对、潜在药源性成分筛选和偏最小二乘法-判别分析(PLS-DA),考察不同提取溶剂对药材溶出成分的影响;建立同步定量分析方法,对药材中绿原酸、连翘酯苷B、毛蕊花糖苷、木犀草苷、槲皮素、木犀草素和芹菜素7种成分的含量进行测定。结果:甲醇、水饱和正丁醇、75%甲醇和50%甲醇能够较为广泛地提取出独一味中的大多数化合物,对所关注的成分有着较高的提取效率。结论:该研究为独一味的提取工艺提供了参考,为其质量控制及药效学研究提供了科学依据。
Objective： To investigate the diverse solvent effect on extraction of chemical components from Lamiophlomis rotata. Methods： Based on LC-MS / MS technique,and chromatographic peak comparison,potential chemical components screening and partial least squares-discriminant analysis（ PLS-DA） were used to establish a quantitation method for simultaneous determination of chlorogenic acid,forsythoside B,verbascoside,luteoloside,quercetin,luteolin and apigenin. Results： According to the results of PLS-DA and quantitative analysis,methanol,water-saturated n-butanol,75% methanol and 50% methanol showed wider extraction range and higher extraction efficiency to the chemical components of Lamiophlomis rotata. Conclusion： This study offeres references to the extraction technology of Lamiophlomis rotata,and promotes the relative pharmacodynamics study of this medical material.
出处
《中药材》
CAS
CSCD
北大核心
2016年第3期571-574,共4页
Journal of Chinese Medicinal Materials
基金
国家科技支撑计划资助项目(2008BAI51B01)
南京中医药大学青年自然科学基金资助项目(13XZR28)
关键词
独一味
提取溶剂
LC-MS/MS
定量分析
质量控制
Lamiophlomis rotata（Benth ） Kudo Solvent LC-MS / MS Quantitative analysis Quality control
