摘要
目的研究趋化因子受体7（CCR7）蛋白和血管内皮生长因子D（VEGF-D）蛋白在乳腺癌癌变过程中,即正常乳腺组织、轻中度导管非典型增生组织、重度导管非典型增生＋导管原位癌组织及乳腺浸润性导管癌组织中的表达及意义。方法采用免疫组化染色法,检测正常乳腺组织（n=20）、乳腺轻中度导管非典型增生组织（n=20）、乳腺重度导管非典型增生＋导管原位癌组织（n=20）及乳腺浸润性导管癌组织（n=73）中CCR7和VEGF-D蛋白的表达情况,并根据D2-40染色结果计算微淋巴管密度（LMVD）。同时分析乳腺癌组织中,CCR7和VEGF-D蛋白表达与乳腺癌临床病理学特征的关系、CCR7和VEGF-D蛋白表达与LMVD值间的关系及CCR7蛋白表达与VEGF-D蛋白表达的关系。结果 1正常乳腺组织组〔CCR7蛋白：0（0/20）,VEGF-D蛋白：5.0%（1/20）〕）、轻中度乳腺导管非典型增生组〔CCR7蛋白：5.0%（1/20）,VEGF-D蛋白：20.0%（4/20）〕）、乳腺重度导管非典型增生＋导管原位癌组〔CCR7蛋白：30.0%（6/20）,VEGF-D蛋白：40.0%（8/20）〕）及乳腺癌组〔CCR7蛋白：47.9%（35/73）,VEGF-D蛋白：57.5%（42/73）〕的CCR7蛋白（χ^2趋势=23.905,P〈0.050）和VEGF-D蛋白（χ^2趋势=22.349,P〈0.050）的表达阳性率逐渐增高。2正常乳腺组织组、乳腺轻中度导管非典型增生组、乳腺重度导管非典型增生＋导管原位癌组及乳腺癌组的LMVD值分别为2.00±1.02、6.70±3.48、9.01±2.13及16.32±4.07,LMVD值逐渐增高,4组间两两比较差异均有统计学意义（P〈0.050）。3在乳腺癌患者中,CCR7蛋白和VEGF-D蛋白的表达均与临床分期、组织学分级、淋巴结转移及人类表皮生长因子2（HER-2）表达有关（P〈0.050）,组织学分级越高、临床分期为Ⅲ＋Ⅳ期（与Ⅰ＋Ⅱ期者相比）、淋巴结转移（与未发生淋巴结转移者相比）及HER-2表达阳性（与HER-2表达阴性者相比）者CCR7蛋白和VEGF
Objective To study the expression and significance of CCR chemokine receptor-7（CCR7） protein and vascular endothelial growth factor-D（VEGF-D） protein in the progression of breast cancer, including normal breast tissue, slight and moderate atypical hyperplasia, severe atypical hyperplasia and intraductal carcinoma in situ, as well as invasive ductal carcinoma. Methods Immunohistochemistry was used to detect the expression of CCR7 and VEGF-D protein in the nomal breast tissue（n=20）, slight and moderate ductal atypical hyperplasia tissue（n=20）, severe atypical hyperplasia and intraductal carcinoma in situ tissue, as well as invasive ductal breast carcinoma tissue（n=73）. In addition, the D2-40 staining was also used to determine lymphatic microvessel density（LMVD）. Meanwhile, the relationship between the expression of the two kinds of protein and clinicopathological factors/LMVD was analyzed by statistical analysis in breast cancer, and the correlation between expression of CCR7 protein and expression of VEGF-D protein was analyzed too. Results 1 The positive rates of CCR7 protein（χ2=23.905,P〈0.050） and VEGF-D protein（χ2 =22.349,P〈0.050） were gradually increased in the normal breast tissue group 〔CCR7 protein： 0（0/20）, VEGF-D protein： 5.0%（1/20）〕,slight and moderate atypical hyperplasia group 〔CCR7 protein： 5.0%（1/20）, VEGF-D protein： 20.0%（4/20）〕, severe atypical hyperplasia and intraductal carcinoma in situ group 〔CCR7 protein： 30.0%（6/20）, VEGF-D protein： 40.0%（8/20）〕,and invasive ductal carcinoma group 〔CCR7 protein： 47.9%（35/73）, VEGF-D protein： 57.5%（42/73）〕. 2 The LMVD value gradually increased in normal breast tissue group（2.00±1.02）, slight and moderate atypical hyperplasia group（6.70±3.48）, severe atypical hyperplasia and intraductal carcinoma in situ group（9.01±2.13）, as well as invasive ductal carcinoma group（16.32±4.07）, there was significant difference between any 2 groups（P〈0.05
出处
《中国普外基础与临床杂志》
CAS
2016年第6期715-721,共7页
Chinese Journal of Bases and Clinics In General Surgery
