摘要
目的阐明2011年-2013年杭州地区致泻性大肠埃希菌(DEC)的流行特征及耐药趋势。方法对1 246例急性腹泻患者粪标本中分离出的大肠埃希菌采用多重PCR技术进行DEC鉴定及分型;纸片扩散法(K-B法)测定DEC对抗菌药物的敏感性。结果 1 246例急性腹泻患者粪标本中共检出DEC 263株,检出率为21.1%,以EAEC(11.8%)为主,其次为ETEC(7.3%)、EPEC(2.0%)。DEC菌株对氨苄西林、复方新诺明、头孢唑啉的耐药率分别为51.3%、43.0%和35.4%,产ESBL菌株的检出率为32.2%,多重耐药菌占44.1%。结论 2011年-2013年来杭州地区DEC检出率较高,对多种抗菌药物的耐药率较高,应加强对DEC的检测及药敏试验,以指导临床合理选用抗生素。
Objective To study the epidemiological characteristics and antibacterial resistance of diarrheagenic Escherichia coli（ DEC） in Hangzhou area from July 2011 to June 2013. Methods The Escherichia coli strains isolated from 1 246 acute diarrhea patients were conducted for DEC identification and typing by multiplex PCR techniques; disk diffusion（ K-B method） was carried out to detect sensitivity of the strains to antibiotics. Results 263 strains of diarrheagenic Escherichia coli were isolated from the fecal specimens of 1 246 patients with acute diarrhea. The detection rate was 21. 1%,with EAEC as the dominant（ 11. 8%）,followed by ETEC（ 7. 3%） and EPEC（ 2. 0%）. The drug resistant rates of DEC strains to AMP,SXT and KZ were51. 3%,43. 0% and 35. 4%. The detection rate of the strains which can produce ESBL was 32. 2%. The multiple antibiotic resistance strains accounted for 44. 1%. Conclusion The detection rate of diarrheagenic Escherichia coli was higher in Hangzhou area from 2011 to 2013,which showed higher multiple antibiotic resistance. Thus,surveillance and drug sensitivity test should be performed on DEC in order to guide the clinical rational use of antibiotics.
出处
《中国卫生检验杂志》
CAS
2016年第11期1655-1657,共3页
Chinese Journal of Health Laboratory Technology
基金
"十二五"科技重大专项浙江及周边省传染病病原谱流行规律课题(2012ZX10004-210)
浙江中医大学校级科研基金项目(2013ZY20)
关键词
腹泻
致泻性大肠埃希菌
多重PCR
毒力基因
血清型
耐药性
Diarrhea
Diarrhea Escherichia coli
Multiplex PCR
Virulence gene
Serotype
Antibacterial resistance
