摘要
目的了解2019—2021年湖北省武汉市腹泻患者来源致泻大肠埃希菌的毒力基因分布、耐药情况以及种群多样性。方法定期收集来自武汉市5家医疗机构的致泻大肠埃希菌菌株并通过生化反应和飞行时间质谱进行确认。对经确认的菌株进行药敏试验以及多重PCR检测毒力基因。采用脉冲场凝胶电泳技术和多位点序列分型技术对收集的菌株进行分子分型。通过聚类比对和构建最小生成树进行同源性分析。结果收集到59株致泻大肠埃希菌,共检出3种基因型别。其中检出产肠毒素大肠埃希菌(ETEC)29株,占比最高为49.15%;肠集聚性大肠埃希菌(EAEC)、肠致病性大肠埃希菌(EPEC)各检出15株,占比均为25.42%。59株致泻性大肠埃希菌对除多黏菌素E外的11种抗生素表现出不同程度的耐药,对氨苄西林(67.80%)、萘啶酸(61.02%)、四环素(45.76%)、复方新诺明(37.29%)、头孢噻肟(30.51%)、氯霉素(13.56%)表现出较高的耐药性。EAEC和EPEC均存在14种不同的脉冲场凝胶电泳带型,均可被分为11种不同的ST型别。ETEC存在28种不同的脉冲场凝胶电泳带型,可被分为10种不同的ST型别,且超过50%的ETEC属于同一种优势克隆复合体CC-10。结论武汉市食源性致泻大肠埃希菌的污染长期存在且耐药情况严重,其中EAEC和EPEC型别多样,ETEC的优势克隆复合体CC-10被广泛检出。
Objective This study aimed to investigate the distribution of virulence genes,antibiotic resistance,and molecular types of diarrheagenic Escherichia coli(DEC)in Wuhan,Hubei Province,China.Methods DEC strains identified through biochemical means and a time-of-flight mass spectrometer were routinely collected from five medical institutions.Multiple polymerase chain reactions and drug sensitivity tests were carried out.The molecular characteristics of DEC strains were determined using pulsed field gel electrophoresis(PFGE)and multi-locus sequence typing.Homology analysis was carried out based on clustering analogy and the minimum spanning tree.Results A total of 59 DEC strains were collected,including 29 strains of enterotoxigenic Escherichia coli(ETEC,49.15%),15 strains of enteroaggregative Escherichia coli(EAEC,25.42%),and 15 strains of enteropathogenic Escherichia coli(EPEC,25.42%).The resistance rates of DECs to the 11 antibiotics(except polymyxin E)varied,and were especially high for the following:ampicillin(67.80%),nalidixic acid(61.02%),tetracycline(45.76%),cotrimoxazole(37.29%),cefotaxime(30.51%),and chloramphenicol(13.56%).As determined by PFGE,both EAEC and EPEC had 14 different bands,belonging to 11 different sequence types(STs);whereas ETEC had 28 different bands belonging to 10 different STs.In addition,more than 50%of STs from ETEC were classified as clonal complexes(CC)-10.Conclusion The contamination of DECs persists in Wuhan,and antimicrobial resistance is at a critical level.The genotypes from EAEC and EPEC are relatively scattered based on molecular detection and typing of DECs.CC-10,the dominant clone complex of ETEC,is widely distributed in Wuhan.
作者
费筱媛
陈小红
赵滢
周军波
黄烈泓
龙一兵
FEI Xiaoyuan;CHEN Xiaohong;ZHAO Ying;ZHOU Junbo;HUANG Liehong;LONG Yibing(Wuhan Center for Disease Control&Prevention,Hubei Wuhan 430000,China;Wuhan Third Hospital,Hubei Wuhan 430000,China)
出处
《中国食品卫生杂志》
CSCD
北大核心
2023年第8期1212-1219,共8页
Chinese Journal of Food Hygiene
基金
湖北省卫生计生委员会科研专项(WJ2018H255)
武汉市卫生健康委医学科研专项(WX21Q13)
关键词
致泻大肠埃希菌
毒力基因
多重耐药
脉冲场凝胶电泳
多位点序列分型
最小生成树
Diarrheagenic Escherichia coli
virulence genes
multidrug resistance
pulsed field gel electrophoresis
multilocus sequence typing
minimum spanning tree
