摘要
目的：探讨慢性乙型肝炎（CHB）患者乙型肝炎病毒脱氧核糖核酸（HBV DNA）与Th1/Th2相关性分析。方法：将135例CHB患者根据HBV DNA水平分为高水平组（HBV DNA为1×10~6~1×10~7拷贝/mL）和低水平组（HBV DNA为1×10~4~1×10~5拷贝/mL）,分别有56例和79例,另选择45例健康志愿者作为对照组,比较各组肝功能指标：谷丙转氨酶（ALT）、谷草转氨酶（AST）、碱性磷酸酶（ALP）,HBV标志物：乙肝表面抗原（HBsAg）、乙肝e抗原（HBeAg）、乙肝e抗体（抗-HBe）,流式细胞仪检测Th1、Th2。结果：3组肝功能指标ALT、AST、ALP比较差异有统计学意义（均P〈0.05）,其中高水平组ALT、AST、ALP显著高于低水平组和对照组,低水平组ALT、AST、ALP显著高于对照组,差异有统计学意义（均P〈0.05）;高水平组HBsAg、HBeAg显著高于低水平组,而抗-HBe显著低于低水平组,差异均有统计学意义（均P〈0.05）;3组Th1、Th2以及Th1/Th2比较差异有统计学意义（均P〈0.05）,其中高水平组和低水平组Th1、Th1/Th2显著低于对照组,Th2显著高于对照组,组间比较差异均有统计学意义（均P〈0.05）;高水平组Th1、Th1/Th2显著低于低水平组,差异有统计学意义（均P〈0.05）,高水平组和低水平组Th2比较差异无统计学意义（P〉0.05）;慢性乙型肝炎患者HBV DNA与Th1、Th1/Th2呈显著负相关（r=-0.391、-0.228,P〈0.05）,与Th2无相关性（r=0.029,P〉0.05）。结论 ：慢性乙型肝炎患者存在Th1/Th2失衡,表现为Th1/Th2降低,且与病毒复制水平有关。
Objective：To investigate the relationship between the hepatitis B virus （HBV）DNA level of chronic hepati-tis B patients and Th1/Th2.Methods：Based on the level of HBV DNA,135 CHB patients were divided into two groups,high level group （the level of HBV DNA 1×10 6 -1×10 7 copies/mL）,and low level group （the level of HBV DNA 1 ×10 4 -1 ×10 5 copies/mL）,with 56 and 79 patients,respectively.In addition,45 healthy volunteers were recruited as control group.Further-more,index of liver function were compared between these three groups,containing glutamic-pyruvic transaminase （ALT）,nbsp;glutamic oxalacetictransaminase （AST）,alkaline phosphatase （ALP）,markers of HBV,including HBsAg,HBeAg,Anti-HBe,Th1 and Th2 by using flow cytometry.Results：ALT,AST and ALP were significantly different between two groups （P〈0.05）.ALT,AST and ALP of high level group were significantly higher than control group and low level group,ALT,AST and ALP of low level group were significantly higher than control group （P 〈0.05）.HBsAg,HBeAg of high level group were significantly higher than low level group,however,anti-HBe was lower in high level group than low level group （P 〈0.05 ）. Th1,Th1/Th2 of high level group were significantly lower than low level group （P 〈0.05 ）.But there was no significantly difference of Th2 level between two groups （P 〉0.05）.There was a negative relationship between HBV DNA level and Th1, Th1/Th2 r =-0.391,-0.228,P 〈0.05）,no relevance with Th2 （r =0.029,P 〉0.05）.Conclusions：Th1/Th2 is imbalanced in chronic hepatitis B patients,displaying low level of Th1/Th2,which is related to level of virus copies.
出处
《海南医学院学报》
CAS
2016年第15期1649-1652,共4页
Journal of Hainan Medical University
基金
青海省科学计划项目(20134-Z-183)~~
