摘要
目的:探讨乙肝病毒(HBV)前S1抗原(Pre-S1Ag)与e抗原(HBeAg)、HBV-DNA含量的相关性,以评价Pre-S1Ag检测在乙型肝炎诊疗中的价值。方法:临床采集乙肝表面抗原阳性标本315例,以ELISA法检测Pre-S1Ag和HBeAg,实时荧光定量PCR(FQ-PCR)法检测HBV-DNA含量,并分析Pre-S1Ag、HBeAg和HBV-DNA的关系。结果:Pre-S1Ag与HBeAg、HBV-DNA之间存在关联性(χ2分别为85.2、159.22,P<0.01)。HBeAg阴性组(e抗体为阳性)Pre-S1Ag的阳性率为37.0%,HBV-DNA的阳性率为43.2%。HBV-DNA阳性(≥500copies/mL)患者Pre-S1Ag阳性率为84.2%,HBeAg阳性率为52.6%,差异有统计学意义(P<0.01),乙肝患者Pre-S1Ag阳性率随HBV-DNA含量的增加而增加。Pre-S1Ag灵敏度、准确度、优势比(84.2%、85.7%、39.1)优于HBeAg(52.6%、69.2%、18.7),特异度、阳性预测值、阳性似然比相近(88.0%vs 94.4%,91.4%vs 93.4%,7.01 vs9.39)。结论:Pre-S1Ag较HBe Ag能更敏感、更准确地反映HBV复制情况,与HBV-DNA的相关程度更高,在乙型肝炎诊疗中有较高价值。
Objective： To explore the relationship among Pre-S1Ag,HBeAg and HBV-DNA of hepatitis B virus（HBV）,and to evaluate the application of Pre-S1 in diagnosis and treatment.Methods： A total of 315 cases with positive HBsAg were selected.Pre-S1 Ag,and HBeAg were determined by ELISA,while the level of HBV-DNA was measured by RT-PCR.And the relationship among Pre-S1Ag,HBeAg and HBV-DNA was analyzed.Results： Pre-S1Ag was associated with HBeAg and HBV-DNA（χ2=85.2 and 159.22,respectively,P〈0.01）. The positive rate of Pre SlAg was 37.0%, and the positive rate of HBV-DNA was 43.2% in HBeAg negative group; while in HBV-DNA positive patients （≥500 copies/ mid, 84.20..4o had the positive rate of Pre-S1Ag, 52.6% had the positive rate of HBeAg. There was signif- icant difference between two groups （P〈0. 01）, and the positive rate of Pre-S1Ag increased as HBV- DNA. The sensitivity, accuracy, and odds ratio of Pre-SIAg were higher than those of HBeAg （4.2% VS 52.6%, 85.7% VS 69.2%, 39.1 VS 18.7）; while specify, positive predictive value and positive likelihood ratio were similar （88.0%VS 94.4% ,91.4% VS 93.40%,7.01 VS 9.39）. Conclusions： Pre-S1Ag is more sensitive and accurate in reflecting the duplication of HBV, and has higher correlativity with HBV DNA. It is valuable in diagnosis and treatment of hepatitis B.
出处
《海南医学院学报》
CAS
2011年第2期190-192,196,共4页
Journal of Hainan Medical University
基金
肇庆市科技局(市级)科技创新立项科研项目(2006E214)~~
