摘要
建立同时检测葡萄汁中3种苯并咪唑类农药（多菌灵、噻菌灵、麦穗宁）残留的分散固相萃取处理结合高效液相色谱方法。样品经乙腈提取,无水MgSO4和NaCl盐析后,用N-丙基乙二胺固相萃取材料分散净化,然后采用高效液相色谱—荧光检测器根据不同的保留时间选择相应的最佳检测波长同时测定3种农药。通过对提取盐、净化剂、色谱条件的优化,方法的加标回收率在85%-115%之间,相对标准偏差在1.95%-9.75%之间,多菌灵、噻菌灵和麦穗宁检出限分别达到17.60、1.20、0.06μg/L。该方法简单、准确、重复性良好,满足葡萄汁中多种苯并咪唑类农药残留同时分析的需求。
An analytical method was established for the simultaneous determination of 3 benzimidazole fungicide（carbendazim, thiabendazole and fuberidazole） residues in grape juices by using high performance liquid chromatographywith fluorescence detector （HPLC-FLD） after quick easy cheap effective rugged safe （QuEChERS） cleanup. The fungicideresidues in grape juice samples were extracted into acetonitrile with the addition of anhydrous MgSO4 and NaCl. Then, thesamples were analyzed after the fungicide residues in the supernatant were purified with N-propyl ethylenediamine （PSA）sorbent. The detection was performed with fluorescence detector under the corresponding optimal wavelengths accordingto different retention times of the three compounds. Under the optimized analytical conditions including extraction salts,purification reagent, mobile phase composition and chromatographic parameters, the recovery of the method for spikedsamples ranged from 85% to 115% with relative standard deviation （RSD） between 1.95% and 9.75%. Meanwhile, the limitsof detection （LODs） for carbendazim, thiabendazole and fuberidazole were 17.60, 1.20 and 0.06 μg/L, respectively. Thesimple and accurate method is appropriate for the simultaneous analysis of the three benzimidazole fungicide residues ingrape juices with good repeatability.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2016年第12期192-196,共5页
Food Science
基金
"十二五"国家科技支撑计划项目(2012BAD29B04)
关键词
葡萄汁
苯并咪唑类农药
QUECHERS
高效液相色谱—荧光检测
grape juice
benzimidazole fungicides
quick easy cheap effective rugged safe （QuEChERS）
high performanceliquid chromatography with fluorescence detector （HPLC-FLD）
