摘要
旨在探索水杨酸(Salicylic acid,SA)信号通路相关的两种酶——苯丙氨酸解氨酶(PAL)和β-1,3-葡聚糖酶(β-1,3-GA)在病烂、盐度、温度逆境和添加SA条件下在龙须菜中的活性变化。结果表明,3组病烂龙须菜中PAL和β-1,3-GA活性分别增加为对照组的1.27-1.42倍和1.26-1.35倍;高盐条件下PAL活性与对照组无显著差别,而β-1,3-GA活性在24 h和48 h分别增加为对照组的1.90倍和1.42倍;高温组PAL和β-1,3-GA活性在24 h均显著升高,分别是对照组的1.25倍和1.27倍;100μmol/L SA添加后PAL和β-1,3-GA的活性升高,但高浓度SA却抑制了两种酶的活性。结果表明,在逆境胁迫下龙须菜中PAL和β-1,3-GA的活性增加与水杨酸的抗逆作用有关,而100μmol/L SA诱导两种酶的效果最显著。
The article mainly studied two enzymes related to Salicylic acid(SA) signaling pathway :phenylalanine ammonia-lyase(PAL)and β-1,3-glucanase(β-1,3-GA)in marine algae Gracilariopsis lemaneiformis,and measured their activity change under sick,high salt,high temperature stresses and addition of SA. Results showed that the activities of PAL and β-1,3-GA in G. lemaneiformis of 3 sick groups increased 1.27-1.42 times and 1.26-1.35 times compared to healthy groups,respectively. For high salt stress,the activities of PAL had no significant difference from the control,whereas β-1,3-GA increased 1.90 times and 1.42 times at 24 h and 48 h compared to the control,respectively. The activities of PAL and β-1,3-GA under high temperature stress increased 1.25 times and 1.27 times compared to the control groups. The activities of PAL and β-1,3-GA increased after adding 100 μmol/L SA,but high concentration of SA inhibited the activities of 2 enzymes. This study indicated that the increases of activities of PAL and β-1,3-GA in G. lemaneiformis might be associated with the SA resistance to environment. In addition,100 μmol/L SA had the most significant effect on the induction of PAL and β-1,3-GA activities.
出处
《生物技术通报》
CAS
CSCD
北大核心
2016年第5期194-199,共6页
Biotechnology Bulletin
基金
国家自然科学基金项目(41376151)
国家教育部博士点博导基金项目(20123305110002)
宁波大学学科项目(xkl141053)
关键词
龙须菜
水杨酸
苯丙氨酸解氨酶
Β-1
3-葡聚糖酶
Gracilariopsis lemaneiformis
salicylic acid
phenylalanine ammonia-lyase
β-1
3-glucanase