摘要
目的研究HMGA1-siRNA基因对甲状腺乳头状癌K1细胞增殖的影响。方法 HMGA1-siRNA组转染HMGA1的小干扰RNA(siRNA);阴性对照组转染HMGA1的无关序列,并转染甲状腺乳头状癌K1细胞。采用CCK-8法检测转染HMGA1-siRNA基因后对K1细胞增殖的影响;RT-PCR法检测正常组、HMGA1-siRNA组和阴性对照组的HMGA1-mRNA表达;Western blot法检测三组的HMGA1蛋白表达;Transwell侵袭实验检测三组HMGA1-siRNA基因转染后K1细胞的侵袭能力。结果 HMGA1-siRNA基因对K1细胞的抑制增殖作用明显,呈现时间-剂量依赖关系;HMGA1-siRNA基因在K1细胞中mRNA和蛋白的表达显著低于正常组和阴性对照组;HMGA1-siRNA组K1细胞侵袭能力显著低于正常组和阴性对照组。结论 siRNA可以沉默HMGA1基因,减缓甲状腺乳头状癌K1细胞增殖。
Objective To study the effect of HMGA1-siRNA gene on proliferation of K1 cells in papillary thyroid carcinoma. Methods Experimental group was transfected with HMGA1 by small interfering RNA（ siRNA）; the negative contrast group was transfected with HMGA1 nonrelevant sequence,and transfected with K1 cells of thyroid papillary carcinoma. The impact on the proliferation of K1 cells after the transfection of HMGA1-siRNA was tested by CCK-8. The HMGA1-mRNA expression in the normal group was tested by RT-PCR. The HMGA1 protein expression in the three groups was tested by Western blot. The invasion of K1 cells transfected with HMGA1-siRNA gene was detected by Transwell invasion assay in three groups. Results The effect of HMGA1-siRNA gene on the proliferation of K1 cells was obvious,which was relevant to time and dosage. The expression of mRNA and protein in K1 cells was significantly lower than that in normal in HMGA1-siRNA cells. The expression of mRNA and protein in K1 cells was significantly lower than that in the normal group and the negative control group. The invasion ability of K1 cells in HMGA1-siRNA group was significantly lower than that in the normal group and the negative control group. Conclusion siRNA can silence HMGA1 gene,and slow down the proliferation of papillary thyroid carcinoma K1 cells.
出处
《安徽医科大学学报》
CAS
北大核心
2016年第6期783-786,共4页
Acta Universitatis Medicinalis Anhui
基金
安徽省高等学校自然科学基金(编号:KJ2015B106by)
