摘要
目的:优选石菖蒲挥发油羟丙基-β-环糊精(HP-β-CD)包合物的制备工艺,为该部位的有效利用提供参考。方法:以挥发油包合率、包合物得率和包合物中α-细辛醚质量分数的综合评分为评价指标,采用星点设计-效应面法考察包合时间、包合温度、主客体配比对石菖蒲挥发油HP-β-CD包合工艺的影响,以红外光谱法(IR)和气相色谱-质谱法(GC-MS)对该包合物进行质量评价。结果:石菖蒲挥发油HP-β-CD包合物的最佳包合工艺为包合时间3.85 h,包合温度30.06℃,HP-β-CD与石菖蒲挥发油投料比8.69∶1。挥发油包合率79.74%,包合物得率87.47%,包合物中α-细辛醚质量分数2.266 mg·g^(-1)。石菖蒲挥发油包合前后GC-MS图谱的整体相似度高。结论:IR和GC-MS分析包合物结果证明包合物制备成功,石菖蒲挥发油在包合前后成分变化不大。星点设计-效应面法适用于石菖蒲挥发油HP-β-CD包合物的工艺优化。
Objective： To optimize the preparation process of inclusion complex of volatile oil from Acori Tatarinowii Rhizoma and hydroxypropyl-β-cyclodextrin（ HP-β-CD）. Method： Taking composite score of inclusion rate of volatile oil,yield of inclusion complex and the content of α-asarone as index,central composite designresponse surface methodology was adopted to optimize inclusion process with inclusion temperature,reaction time,ratio of HP-β-CD and volatile-oil as factors. The quality of inclusion complex was evaluated by infrared spectroscopy（ IR） and GC-MS. Result： The best process was as follows,inclusion time was 3. 85 h,inclusion temperature was 30. 06 ℃,ratio of HP-β-CD and volatile-oil was 8. 69 ∶ 1. Under these conditions,inclusion rate of volatile oil was 79. 74%, yield of inclusion complex was 87. 47%, the content of α-asarone in inclusion complex was 2. 266 mg·g^-1. After inclusion,similarities of GC-MS spectra of volatile oil was high. Conclusion：The characterization results of IR and GC-MS proved the generation of inclusion complex. Central composite design-response surface methodology is suitable for optimizing inclusion process of inclusion complex of volatile oil from Acori Tatarinowii Rhizoma and HP-β-CD.
出处
《中国实验方剂学杂志》
CAS
CSCD
北大核心
2016年第11期20-24,共5页
Chinese Journal of Experimental Traditional Medical Formulae
基金
中国中医科学院自主选题(Z02063)
北京市共建项目专项
关键词
石菖蒲
挥发油
羟丙基-Β-环糊精
Α-细辛醚
红外光谱法
气相色谱-质谱
Acori Tatarinowii Rhizoma
volatile oil
hydroxypropyl-β-cyclodextrin
α-asarone
infrared spectroscopy
gas chromatography-mass spectrometry
