摘要
目的:建立侧柏炭的HPLC指纹图谱分析方法。方法:采用Lichrospher C18色谱柱(250mm×4.6mm5μm),流动相为0.5‰三氟乙酸水溶液-甲醇,梯度洗脱,检测波长375nm。采用《中药色谱指纹图谱相似度评价系统(2004A)版》,计算各被测样品的HPLC指纹图谱的整体相似度。结果:共有14个共有峰,10批被测样品的色谱指纹图谱的整体相似度在0.178～0.963,并得出了侧柏炭的对照指纹图谱。结论:该指纹图谱方法简便、重现性好,可专属性地研究侧柏炭的指纹图谱,为有效地控制该饮片的内在质量提供了科学依据。
AIM：To establish the fingerprint for Cacumen Platycladi （carbonized） by HPLC. METHODS：The column of Lichrospher C 18 （250 mm×4.6 mm 5 μm）was used. The mobile phase consisted of 0.5‰ trifluoroactic acid-methanol with gradient elution. The detective wavelength was at 375 nm,and the flow rate was 1.0 mL/min. Different habitats were compared by Similarity Evaluation System for Chromatographic Fingerprint of CMM Version 2004A. RESULTS：The fingerprint consisted of 14 common peaks. The mutual mode of HPLC fingerprints was set up and the similarity of the crude drugs was in the range of 0. 178 - 0. 963. The standard HPLC fingerprint of Cacumen Platycladi （carbonized） was established too. CONCLUSION： This method is accurate and reliable and provides a scientific basis for the quality control of Cacumen Platycladi （carbonized）.
出处
《中成药》
CAS
CSCD
北大核心
2009年第12期1808-1810,共3页
Chinese Traditional Patent Medicine
基金
国家"十一五"科技攻关项目(2006BAI09B06-02)
