摘要
目的:建立大黄饮片MEKC-DAD指纹图谱分析方法,并对大黄及其炮制品的指纹谱进行比较。方法:选择胶束电动毛细管电泳分离模式,以25 mmol.L-1硼砂-25 mmol.L-1SDS-10%乙腈(pH10.90)为运行缓冲液,分离电压12 kV,以大黄酸为参照物(IS),测定其指纹图谱,并作模糊聚类法分析和相似度评价。结果:初步建立了以11个共有峰为特征指纹信息的10批大黄地产药材MEKC-DAD指纹图谱;发现少数产地大黄药材的指纹图谱有一定差异,生品与其炮制品的指纹谱中共有峰相对峰面积差异显著。结论:方法准确可靠,重复性好,可作为大黄饮片内在质量评价的依据。
Objective:To establish the analytical method for the fingerprint of Radix et Rhizoma Rhei by MEKC-DAD and compare the fingerprints of Radix et Rhizoma Rhei and its processed products.Method:Based on the mode of micellar electrokinetic chromatography,25 mmol·L-1 borax-25 mmol·L-1 SDS-10% acetonitrile was selected for the running buffer(pH 9.2).The separation voltage was 12 kV and the detection wavelength was set at 254 nm.Rhein was used as a reference standard,the chromatographic fingerprint was determined v...
出处
《中国中药杂志》
CAS
CSCD
北大核心
2009年第23期3034-3038,共5页
China Journal of Chinese Materia Medica
基金
江苏省中药炮制重点实验室开放式课题(ZYPZ007)
