摘要
为了解2013年安阳地区手足口病病原谱组成特点及柯萨奇病毒A6(CV-A6)VP1基因特征,采用实时荧光逆转录PCR方法对全年采集的临床手足口病例的479份粪便标本进行检测和分型,统计各型肠道病毒阳性标本数量及比例。对10份CV-A6阳性标本VP1基因进行扩增测序以获得全序列。使用BIOEDIT 7.2和MEGA 5.1软件对获得序列和NCBI数据库中检索到的参考序列进行VP1基因核苷酸、氨基酸相似性及系统发育分析。研究结果显示,检出肠道病毒阳性标本共429份,其中CV-A6阳性标本为90份,占全部阳性标本的比例为20.98%,这是安阳地区首次记录非CV-A16和EV-A71型肠道病毒列居手足口病病原谱第二位,成为手足口病主要病原之一。将成功获得的10条VP1序列与CV-A6原型株Gdula比较,发现安阳序列与Gdula VP1基因的核苷酸、氨基酸序列差异皆较大。与河南历史株HN421(2011)比较,发现有4条本地序列与HN421的核苷酸、氨基酸序列差异较大,其余6条与HN421差异较小。系统发育树显示,全部49条CV-A6序列共形成A、B、C、D四个分支。其中D分支可进一步分为D1和D2两个亚分支。安阳地区获得的10条CV-A6序列中,有6条属于D1亚分支,4条属于D2亚分支。
This study aims to investigate the etiologic spectrum features of hand,foot and mouth disease（HFMD）and the VP1 region of coxsackievirus A6（CV-A6）in the Anyang area of China during 2013.A total of 479 HFMD fecal specimens were identified using real-time RT-PCR.The positive specimens of the different serotypes and the proportion of each serotype were calculated.A total of 10 positive of CV-A6 specimens were amplified and sequenced to obtain the profile of the VP1 region.Based on the data of the VP1 sequences from the Anyang strains and other referenced strains deposited in the NCBI database,the similarities of the nucleotide and amino-acid sequences for the VP1 region,as well as phylogenetic analyses were performed by the BIOEIDT 7.2and MEGA 5.1software packages.The results revealed that 429 positive specimens of enterovirus,including 90 positive specimens of CV-A6 were identified.The CV-A6 specimens accounted for 20.98%of the total positive specimens.CV-A6 had become the second preponderant pathogen of the HFMD in the Anyang area during 2013.It was the first record of an enterovirus serotype of which was neither CV-A16 nor EV-A71,had become one of the primary pathogens associated with HFMD in the region.A great diversity of the nucleotide and amino-acid sequences between 10 Anyang strains and a prototype strain（Gdula）was found.Compared with the Henan strain（HN421）,four Anyang sequences with nucleotide and amino-acid sequences exhibited greater diversity than the other six Anyang sequences.The phylogenetic analysis showed that all of 49CV-A6 strains could be clustered into four branches.Branch D could be further clustered into two sub-branches;6 Anyang sequences belonged to sub-branch D1,and the other four belonged to sub-branch D2.
出处
《病毒学报》
CAS
CSCD
北大核心
2016年第3期324-330,共7页
Chinese Journal of Virology
基金
河南省科技攻关计划项目(122102310268)
河南省医学科技重大攻关项目(201001015)
河南省卫生科技创新型人才工程中青年科技创新人才
河南省安阳市科技攻关项目(2013042)
关键词
手足口病
肠道病毒
柯萨奇病毒A6
系统发育树
Hand
foot and mouth disease
Enterovirus
Coxsackievirus A6
Phylogenetic tree
