摘要
目的了解烟台地区手足口病合并脑炎病原谱及其基因学特征。方法采集烟台地区手足口病合并脑炎患者粪便及脑脊液标本，通过细胞培养分离病毒，实时荧光PCR鉴定病毒型别，RT-PCR扩增VP1区基因部分序列并测序，进行序列分析。结果10份粪便标本分离病毒3株，均为EV71。与C4a型代表株核苷酸同源性为98％-99％，氨基酸同源性98．90％～99．45％。系统发生树中，烟台分离株与C4亚型C4a簇代表株位于同一分支。结论烟台地区手足口病合并脑炎病原主要为EV71，属于C4亚型C4a簇。
Objective To learn about the pathogen spectrum and genetic characterization of HFMD with encephalitis in Yantai city. Methods Stool samples and cerebrospinal fluids （ CSF ） collected from HFMD with encephalitis cases in Yantai. Virus were isolated from stool samples and identified by fluorescence reverse transcription--polymerase chain reaction. The VP1 region was amplified and sequenced from positive specimens. Genetic characterization was identified by sequence analysis. Results Getting 3 virus strains from 10 stool specimens and all of them belong to EVT1. The nucleotide and amino acid homogeneity with the representative isolates of C4a were 98%-99% and 98.90%-99.45% respectively. Conclusion The pathogen of HFMD with encephalitis in Yantai city were mainly EV71 wich belong to subgenogroup C4 cluster C4a.
出处
《中华实验和临床病毒学杂志》
CAS
CSCD
2012年第5期338-340,共3页
Chinese Journal of Experimental and Clinical Virology
关键词
手足口病
脑炎
肠道病毒属
序列分析
Hand,foot and mouth disease
Encephalitis
Enterovirus
Sequence analysis
