摘要
目的了解新疆生产建设兵团地区柯萨奇病毒A6(CVA6)的流行特点并对其基因特征进行分析。方法对2014-2016年新疆生产建设兵团地区收集的手足口病非肠道病毒71型(EV71)非柯萨奇病毒A16型(CVA16)阳性标本进行CVA6荧光定量PCR检测,采用RT-PCR扩增CVA6阳性标本中VP1编码区基因,并进行核苷酸序列测定与分析;利用MEGA6.06与GenBank代表株进行核苷酸同源性分析并构建系统进化树。结果2014-2016年采集的CVA6阳性标占在非EV71非CVA16阳性标本的82.39%,68株CVA6流行株之间VP1区核苷酸和氨基酸序列同源性分别为92.5%~100.0%和98.9%~100.0%,系统发育树显示97条CVA6序列共形成A-D 4个分支,其中D分支进一步分为D1-D3 3个亚分支。CVA6流行株中的5株处于D2亚分支,63株处于D3亚分支。结论新疆生产建设兵团地区2014-2016年手足口病非EV71非CVA16肠道病毒以CVA6为主,基于VP1系统发育分析分为A-D 4个分支,D分支中的D2和D3亚分支并存,其中D3亚分支是优势流行株。
Objective To ascertain outbreaks and genetic characteristics of coxsackievirus A6（CVA6）prevalent among the Xinjiang Production and Construction Corps.. Methods Samples were previously collected from patients with hand,foot,and mouth disease in the Xinjiang Production and Construction Corps who tested negative for enterovirus 71（EV71）and coxsackievirus A16（CVA16）from 2014 to 2016.Real-time PCR was used to screen for CVA6 in those samples.The VP1 coding region of CVA6 strains was amplified with RT-PCR,and its nucleotides were sequenced and analyzed.These strains and representative strains downloaded from GenBank were subjected to phylogenetic analysis and sequence alignment using the software MEGA6.06. Results Results indicated that 82.39% of samples that were negative for EV71 and CVA16 were positive for CVA6.The VP1 coding region of 68 CVA6 strains had a nucleotide sequence similarity of 92.5%-100.0% and an amino acid similarity of 98.9%-100.0%.Phylogenetic analysis indicated that 97 sequences of the VP1 coding region of CVA6 were all clustered in 4 branches,designated A,B,C,and D.Branch D was further divided into three sub-branches（D1-D3）.The 4 clusters had nucleotide similarity ranging from 15.5%to 26.0%.Of 68 strains found among the Xinjiang Production and Construction Corps,5 were clustered in the D2 sub-branch and 63 strains were clustered in the D3 sub-branch. Conclusion Samples collected from the Xinjiang Production and Construction Corps from 2014 to 2016 that that were negative for EV71 and CVA16 tested positive primarily for CVA6.Based on aphylogenetic analysis of the VP1 coding region,the CVA6 strains were clustered into A,B,C,and D branches.Strains occupying the D2 and D3 sub-branches were prevalent among the Xinjiang Production and Construction Corps.Strains occupying the D3 sub-branch were predominant.
作者
郭景霞
李红叶
刘玉霞
王建平
李凡卡
GUO Jing-xia, LI Hong-ye, LIU Yu-xia, WANG Jian-ping, LI Fan-ka(Center for Disease Control and Prevention, Xinjiang Production and Construction Corps, Urumqi 830002, Chin)
出处
《中国病原生物学杂志》
CSCD
北大核心
2018年第2期181-184,188,共5页
Journal of Pathogen Biology
基金
2014年兵团卫生科技项目(兵卫发[2014]58号)
