摘要
以煎炸废油作为发酵过程中的碳源,对杀虫贪铜菌发酵生产PHA过程的发酵特性进行了研究。考察了发酵过程中初使pH值、摇瓶装液量、培养温度、摇床转速、接种量和废油添加量六个因素与细胞干重及PHA产量之间的关系。实验结果表明:以餐厨废油作为碳源物质发酵制备PHA是可行的,并得到发酵优化条件为:初使pH值为7.5、装液量100mL/250mL、培养温度28℃、接种量3%、转速160r/min、废油添加量25mL/L,在此条件下菌株合成PHA能力最佳。
Waste fried oil was used as carbon source in the process of PHA fermentation.The effects of six factors of fermentation condition on PHA synthetic quantity and cell weight were investigated.Fermentation condition factors include initial pH value,fluid volume per flask,culture temperature,shaking speed,inoculation amount and waste fried oil volume.The result shows that,at the condition of pH 7.5,100 mL fluid medium per 250 mL conical flask,28 ℃,3 %inoculation amount,shaking speed 160r/min and 25mL/L waste fried oil,the yield of PHA reached a maximum value.
出处
《绿色科技》
2016年第10期65-67,71,共4页
Journal of Green Science and Technology
基金
北京市自然科学基金(编号:8144041)
"十二五"国家科技支撑计划(编号:2012BAC25B01
2014BAC27B01-03)
关键词
聚羟基脂肪酸酯
煎炸废油
发酵条件
Polyhydroxyalkanoates
Waste fried oil
Fermentation condition factors
