摘要
从被废油长期污染的土壤中分离出一株酶活力大于5 U/mL的菌株,经16S rRNA鉴定,确定为铜绿假单胞菌属,命名为Pseudomonas aeruginosa B6。经发酵优化,取得其最佳发酵培养基与条件:葡萄糖0.4%、酵母粉0.7%、硫酸铵0.7%、七水硫酸镁0.1%、磷酸氢二钾0.1%;接种量为9.5%;起始pH值为6.5;温度为30℃,为脂肪酶发酵生产奠定了基础。
A wild lipase-producing strain was isolated from long waste-oil-polluted soil with enzyme activity of higher than 5 U/mL and identified as Pseudomonas aeruginosa by the identification of 16S rRNA,and was named B6.The fermentation condition of lipase production strain were optimized and the effect of carbon sources,nitrogen sources,and inoculum size on the lipase production were studied.The result showed that the optimal medium composition for lipase production was as follows（%,w/v）： glucose 0.4,yeast extract 0.7,（NH4）SO4·0.7,MgSO47H2O 0.1,K2HPO4 0.1.The optimal initial pQH and temperature of fermentation medium was 6.5 and 30℃.This experiment lay the foundation for the further study of lipase production fermentation.
出处
《广东农业科学》
CAS
CSCD
北大核心
2012年第17期100-102,共3页
Guangdong Agricultural Sciences
基金
广东省大学生创新性实验计划项目(1134710023)
仲恺农业工程学院基础生物学与生物技术实验教学示范中心开放基金(TP2010003
IP2010001)
关键词
脂肪酶
假单胞菌
分离鉴定
发酵
lipase
pseudomonas
isolation and identification
fermentation
