摘要
以茂原链霉菌（Streptomyces mobaraense：DSM40587）为生产菌株,以提高谷氨酰胺转胺酶（TGase）的产量为主要研究目标,通过摇瓶发酵条件的研究,提高了酶的产量,缩短了发酵时间.茂原链霉菌摇瓶生产TGase最终发酵条件为：种龄48 h,接种量8％～12％;培养基组成为：聚蛋白胨30 g/L,可溶性淀粉30 g/L,磷酸氢二钾2 g/L,七水硫酸镁1 g/L,pH=7.0;发酵时间为120 h.
Streptomyces mobaraense （DSM40587） was used for producing transglutaminase, and improving the yield. By studying on the con- dition of shake-flask culture, we improved the yield of transglutaminase and shorted the culture time. The inoculation condition of Strepto- myces mobaraense cultured by shake-flask was 8%-12% inoculation and the stain were cultured for 48 h. The fermentation condition was Er- leumeyer flasks （250 mL） filled with 50 ml of the medium composed of 30 g/L polypeptone, 30 g/L soluble starch, 2 g/L K2HPO4, 1 g/L MgSO4.7H20, pH=7.0, shaken at 200 rpm for 120 h.
出处
《中国乳品工业》
CAS
北大核心
2014年第7期16-19,共4页
China Dairy Industry
基金
国家自然科学基金项目(No.31301545)
黑龙江省博士后基金(LBH-Z13042)
关键词
茂原链霉菌
谷氨酰胺转胺酶
发酵条件
产量
Streptomyces mobaraense
transglutaminase
fermentation condition
yield
