摘要
目的观察外源性Apelin-13对自发性高血压大鼠(SHR)心肌肥厚的影响,并初步探讨其作用机制。方法将24只20周龄雄性SHR随机分为空白对照组(NTC)、硝苯地平组(NIF)、Apelin-13组(Apelin-13),每组8只。干预4周后,测定无创尾动脉血压及左室质量指数;超声心动图评估心肌肥厚和心功能;HE染色评价心肌细胞及排列情况;Western blot法检测心肌组织Apelin-13、APJ、ANP、IKBα和NF-κB蛋白表达。结果与NTC组和NIF组相比,Apelin-13组的收缩压(SBP)、左心室重量/体重(LVW/BW)、舒张期室间隔厚度(IVSD)和心肌组织ANP降低(P<0.05),左室舒张末期内径(LVEDd)、左心室射血分数(EF)和左心室短轴缩短率(FS)升高(P<0.05);Apelin-13组的心肌组织Apelin-13、APJ和IKBα蛋白表达升高(P<0.05),NF-κB蛋白表达降低(P<0.05)。HE染色提示NIF组和Apelin-13组干预后心肌细胞肥大改善、排列趋向整齐。结论 Apelin-13可以减轻SHR心肌肥厚和改善心功能,其机制可能与下调NF-κB信号通路相关。
Objective To observe the effects of Apelin- 13 on myocardial hypertrophy in the spontaneously hypertensive rats（ SHR）,and to explore preliminarily its possible mechanism. Methods 24 male SHR only 20 weeks old were randomly divided into no- treatment control group（ NTC）,nifedipine group（ NIF）,Apelin- 13 group（ Apelin- 13）,with 8 rats in each group. After treating for 4 weeks,noninvasive tail arterial blood pressure and left ventricular weight / body weight were separately measured. Echocardiography system was used to assess myocardial hypertrophy and cardiac function. HE staining was used to evaluate myocardial cells and arrangement. The expression level of Apelin- 13,APJ,ANP,IKBα,NF- κB in the myocardium was detected by Western blot. Results Compared to the NTC and NIF group,the level of SBP,LVW /BW,IVSD and myocardial ANP in Apelin- 13 group decreased obviously（ P〈0. 05）. The the LVEDd,EF and FS increased significantly（ P〈0. 05）. Besides,the expression of myocardial Apelin- 13,APJ and IKBαin Apelin- 13 group increased significantly（ P〈0. 05）,the expression of myocardial NF- κB decreased obviously（ P〈0. 05）. HE staining indicated that cardiomyocytes hypertrophy improved and the myocardial arranged neatly in the NIF and Apelin- 13 group. Conclusion The treatment of Apelin- 13 can reduce myocardial hypertrophy and improve cardiac function in SHR by down- regulating the NF- κB signaling pathways.
出处
《临床和实验医学杂志》
2016年第9期825-829,共5页
Journal of Clinical and Experimental Medicine
