摘要
以本溪市马铃薯研究所保存的种质资源和自选品系为试验材料,进行茎尖剥离。通过添加不同种类、不同浓度激素,研究其对马铃薯茎尖培养成苗率的影响。结果表明,添加ZT处理的成苗率没有添加6-BA处理的成苗率高,6-BA和GA3浓度为0.5 mg/L的处理成苗率高于其他处理。适宜进行茎尖剥离的激素配比为MS+0.5 mg/L 6-BA+0.01 mg/L NAA+0.5 mg/L GA3。
Germplasm resources and clones preserved by Benxi Potato Research Institute were used as test materialsfor shoot tip culture. The effect of hormone on plantlet regeneration percentage of potato shoot tip culture was studiedusing different ratios and types of hormone. The results showed that plantlet regeneration percentage of treatments addingZT were lower than those adding 6-BA. The plantlet regeneration percentage of treatment with 0.5 mg/L 6-BA and 0.5 mg/LGA3 was the highest among six treatments. The best ratio suitable for potato shoot tip culture was MS ＋ 0.5 mg/L 6-BA ＋0.01 mg/L NAA ＋ 0.5 mg/L GA3.
出处
《中国马铃薯》
2016年第3期140-143,共4页
Chinese Potato Journal
基金
现代农业产业技术体系专项资金(CARS-10)
关键词
马铃薯
激素配比
茎尖剥离
成苗率
potato
hormone ratio
shoot tip culture
plantlet regeneration percentage
