摘要
为探索马铃薯脱毒的最佳方法,提高马铃薯脱毒效率及脱毒苗成苗率,采用超低温疗法+茎尖剥离技术和热处理法+茎尖剥离技术2种脱毒方法进行脱毒效果的比较研究。结果表明:相比于超低温疗法,热处理法处理的马铃薯成苗率和脱毒效率更高,其最佳处理方式为38℃温度条件下热处理6 h后再进行茎尖剥离;同时,在该处理条件下,进一步探索了缩短脱毒苗培养周期的最适激素配比,并发现MS培养基中添加0.50 mg/L GA3+0.50 mg/L 6-BA+0.01 mg/L NAA激素后茎尖成苗率较高,达到84.62%。
In order to explore the best method for potato detoxification and improve the potato detoxification efficiency, this study compared two detoxification methods, namely ultra-low temperature therapy with shoot tip stripping technology and heat treatment with shoot tip stripping technology to find the better detoxification effect.The results showed that compared with the ultra-low temperature treatment, the heat treatment method had the higher seedling rate and detoxification efficiency.In addition, we further explored the optimal hormone ratio to shorten the cultivating period of detoxified seedlings and found that MS medium supplemented with 0.50 mg/L GA3+0.50 mg/L 6-BA+0.01 mg/L NAA could have the higher shoot tip seedling rate, which was 84.62%.
作者
黄莓
唐雪梅
凌思琦
颜朗
赖先军
HUANG Mei;TANG Xuemei;LING Siqi;YAN Lang;LAI Xianjun(Panxi Crops Research and Utilization Key Laboratory of Sichuan Province,Xichang University,Xichang,Sichuan 615013,China)
出处
《西昌学院学报（自然科学版）》
2022年第2期1-4,12,共5页
Journal of Xichang University(Natural Science Edition)
基金
国家自然科学基金项目(32060720)
西昌学院高层次人才引进科研启动项目(50190005)。
关键词
马铃薯
脱毒
超低温疗法
热处理
激素配比
potato
detoxification
ultra-low temperature therapy
heat treatment
hormone formula
