摘要
以马拉硫磷和百菌清为胁迫因子,本文研究了2种污染物暴露下斑马鱼行为强度的变化。首先通过急性毒性试验确定了2种农药对斑马鱼24 h、48 h、72 h、96 h的半致死浓度(LC50),其次以LC50-48 h为1个染毒单位(TU),在0.01 TU、0.1 TU、0.5 TU、1 TU、2 TU 5个条件下,本文开展了斑马鱼行为学研究。结果显示,48 h马拉硫磷暴露斑马鱼的LC50为5 mg·L^(-1),百菌清暴露斑马鱼的LC50为0.01 mg·L^(-1)。随着污染物浓度增大,综合行为强度变化幅度增大。在设置的暴露浓度下,斑马鱼的行为强度在急剧增大或减小后都出现了明显的调节适应过程,基本符合环境压力模型。通过比较在不同频率下的影响强度,马拉硫磷和百菌清暴露斑马鱼摆鳍所受影响最大。本研究提示,水质监测中可增加斑马鱼摆鳍观察指标。
Behavioral changes of zebrafish exposed to malathion and chlorothalonil were studied in this paper. After treatment for 24 h,48 h,72 h and 96 h,median lethal concentration was determined by acute toxicity test. Employed LC50-48 has a toxic unit（ TU,toxicity unit）,behavioral test of zebrafish exposed to 0. 01 TU,0. 1 TU,0. 5TU,1 TU and 2 TU were also implemented. Result from this study demonstrated that LC50 values of the adult zebrafish exposed to malathion and chlorothalonil for 48 h were 5 mg·L^-1and 0. 01 mg·L^-1respectively. Associated with up-regulation of pollutant concentrations,zebrafish behavior strength changes appeared an increasing trend.And there was a regulation and adaptation process after dramatically increasing or decreasing of its behavior strength,which was accorded with environmental stress model. According to multiple comparisons of behavioral in-dicators at different frequencies,zebrafish fins swing was observed to be the most significantly influenced by malathion and chlorothalonil. This study suggests that the monitoring of zebrafish fins swing may be used as a parameter in water quality monitoring.
出处
《生态毒理学报》
CAS
CSCD
北大核心
2016年第1期369-374,共6页
Asian Journal of Ecotoxicology
基金
山东省自然科学基金项目(ZR2014CP019)
国家水体污染控制与治理科技重大专项"南水北调山东受水区饮用水安全保障技术研究与综合示范"课题(2012ZX07404-003)
直饮水科技惠民示范工程(2013GS370202)
关键词
马拉硫磷
百菌清
斑马鱼
行为变化
malathion
chlorothalonil
zebrafish
behavioral changes
