摘要
基于斑马鱼模型探究双酚芴(9,9-bis(4-hydroxyphenyl)fluorine,BHPF)诱导的神经毒性及作用机制。通过黑白箱测试、新型水槽测试和T迷宫测试开展BHPF对斑马鱼神经行为学影响的检测,通过实时荧光定量PCR(quantitative real-time PCR,qRT-PCR)研究BHPF神经毒性的作用机制。黑白箱测试结果显示,BHPF造成斑马鱼运动能力异常;新型水槽测试结果显示,BHPF导致斑马鱼探索行为异常;T迷宫测试结果显示,BHPF对斑马鱼学习记忆能力产生影响。qRT-PCR结果显示,BHPF可以造成斑马鱼神经发育、自噬和凋亡相关基因的异常表达。本研究揭示了BHPF可以造成斑马鱼运动能力和探索行为异常,影响学习记忆功能。BHPF通过影响神经发育、自噬和凋亡等途径诱导神经毒性。
Investigating the neurotoxicity induced by 9,9-bis(4-hydroxyphenyl)fluorene(BHPF)and its mechanism based on zebrafish model.Using zebrafish as a model,the neurobehavioral study of BHPF was carried out by using light/dark tests,novel tank tests,and T-maze tests.Quantitative real-time PCR(qRT-PCR)was used to investigate the mechanism of neurotoxicity induced by BHPF.Light/dark tests showed that BHPF caused abnormal locomotor ability of zebrafish.Novel tank tests indicated that BHPF induced abnormal exploration behavior of zebrafish.T-maze tests revealed that BHPF had adverse effects on learning and memory ability of zebrafish.qRT-PCR results showed that BHPF could cause abnormal expression of genes related to neurodevelopment,autophagy,and apoptosis.This study revealed that BHPF causes abnormal locomotor ability and exploration behavior in zebrafish,as well as injured learning and memory ability.BHPF induces neurotoxicity by affecting neurodevelopment,autophagy,and apoptosis.
作者
张鹏宇
王宝堃
刘可春
靳梦
Zhang Pengyu;Wang Baokun;Liu Kechun;Jin Meng(Institute of Biology,Qilu University of Technology(Shandong Academy of Sciences),Key Laboratory of Drug Screening Technology of Shandong Academy of Sciences,Jinan 250103,China;College of Psychology and Mental Health,North China University of Science and Technology,Tangshan 063200,China)
出处
《生态毒理学报》
CAS
CSCD
北大核心
2022年第3期157-166,共10页
Asian Journal of Ecotoxicology
基金
山东省“外专双百计划”(WST2020008)
齐鲁工业大学(山东省科学院)科教产融合创新试点工程项目(2020KJC-ZD08,2020KJC-GH02,2020KJC-ZD10)。
关键词
双酚芴
斑马鱼
神经行为学
神经发育
自噬
急性毒性
bisphenol fluorene
zebrafish
neurological behavior
neurodevelopment
autophagy
acute toxicity
