摘要
目的建立一种简易的疟色素提取方法,提高拉曼光谱技术检测疟疾的灵敏度。方法利用皂素的破膜特性提取疟色素,采用显微共聚焦拉曼光谱仪进行检测,通过软件处理拉曼信号,用化学计量法比对分析。结果疟色素提取物与阳性对照的拉曼光谱共有峰的相对位移频率、相对峰强度具有较好的一致性,与阴性对照没有相关性,检测的最小虫血率为0.1%。结论皂素可用于疟色素提取。疟色素提取物拉曼光谱检测能有效区分疟原虫感染红细胞与正常红细胞。
Objective To establish a simple hemozoin extract method and to increase detection sensitivity of malaria by Raman pectroscopy. Methods Hemozoin was collected by saponin solution with its membrane rupture character-istics. Raman signals were detected by confocal Raman spectroscopy and analyzed with chemical metrology method.Results The Raman shift and relative peak intensity of extracted hemozoin showed good consistency with positive control（β-hematin） and inconsistent with negative control（normal red blood cells）. The minimum infection rate of malaria detection was 0.1%. Conclusion Saponin could be used for hemozoin extraction. The Raman spectrum of hemozoin extracts were found characteristic peaks and could be distinguished Plasmodium infected red blood cells with normal red blood cells effectively.
出处
《中国国境卫生检疫杂志》
CAS
2016年第2期91-94,107,共5页
Chinese Journal of Frontier Health and Quarantine
基金
国家质检总局科研基金项目(2014IK054)
关键词
皂素
拉曼光谱技术
Saponin
Ramab spectroscopy
