摘要
目的:考察炮制前后知母(Anemarrhena asphodeioidesBge.)中黄酮类成分芒果苷和新芒果苷的含量变化。方法:采用HPLC法测定知母、炒知母中芒果苷和新芒果苷的含量,流动相为乙腈-25 mmol.L-1磷酸二氢钾缓冲液(pH 3.0)梯度洗脱。应用中药色谱指纹图谱相似度评价系统2004 A版(国家药典委员会)对3个批号的知母炮制前后的色谱图进行评价。结果:3个批号的知母炮制前后芒果苷的含量升高,而新芒果苷的含量下降;炮制前后图谱的相似度均有所下降(<1)。结论:炮制后知母中黄酮类成分芒果苷和新芒果苷的含量发生了变化。
Objective：To determine the changes of mangiferin and neomangiferin contents in Rhizoma Anemarrhenae before and after processing. Methods： High performance liquid chromatography （HPLC） was used to determine the changes of mangiferin and neomangiferin contents in Rhizoma Anemarrhenae before and after processing. The mobile phase was ACN： 25 mmol/L KH2 PO4 （pH 3.0）, gradient elution. The Similarity Evaluation System for Chromatographic Fingerprint of TCM （2004 A edition） was used to evaluate the similarity of the 3 batches of Rhizoma Anemarrhenae before and after processing. Results： Mangiferin in the 3 batches was upregulated and neomangiferin was downregulated afer prosessing. The similarity of the 3 batches was all decreased（〈 1）. Conclusion： Processing changes the contents of mangiferin and neomangiferin in Rhizoma Anernarrhenae.
出处
《第二军医大学学报》
CAS
CSCD
北大核心
2006年第5期528-530,共3页
Academic Journal of Second Military Medical University
基金
上海市科委科技攻关(02419125)
上海市科委重大项目(2403DZ19548)~~
关键词
炮制
知母
芒果苷
新芒果苷
processing
anemarrhenae
mangiferin
neomangiferin
