摘要
目的:研究紫苏叶提取物(PFE)对高脂饲料诱导肥胖模型小鼠脂肪组织中脂肪生成及脂质合成相关基因表达的影响,探讨PFE影响脂肪脂质代谢的可能机制。方法:5周龄的雄性ICR小鼠,适应性喂养1周后,随机分为正常饲料(RD)组和高脂饲料(HFD)组,HFD+PFE(150、300mg/kg)组,每天灌胃给药1次,RD组灌服等量蒸馏水,每周测2次体质量,连续4周。实验结束后,分别称量内脏脂肪和附睾脂肪重量;检测血浆血糖、胰岛素、HOMA-IR指数、甘油三酯(TG)、胆固醇(TC)、非游离脂肪酸(NEFA)、谷草转氨酶(AST)和谷丙转氨酶(ALT)。用Western blot法和RT-PCR法检测过氧化物酶体增殖物激活受体-γ(PPAR-γ)和CAAT/增强子结合蛋白α(C/EBPα)的表达。此外,检测脂肪合成转录因子SREBP1及其靶基因(FAS、SCD1、GPAT)的基因表达。结果:与HFD组比较,治疗4周后,PFE显著降低了小鼠体质量、内脏脂肪量及附睾脂肪量,血糖、胰岛素水平均显著下降,HOMA-IR也得到改善。血脂(TG、TC、NEFA)和肝功(s AST和s ALT)在给PFE后均显著降低。与RD组比较,脂肪生成转录因子PPAR-γ、C/EBPα和脂肪合成转录因子SREBP1及其靶基因在HFD组增强,而在PFE组上述基因表达均显著被抑制。结论:PFE通过抑制脂肪生成转录因子PPAR-γ、C/EBPα和脂肪合成转录因子SREBP1及其靶基因的表达来降低脂肪和体质量,调节脂肪组织脂质代谢。
Objective: To study the effects of Perillae folium extract(PFE) on thelipogenesis and lipid synthesis related gene expression in the adipose tissue of obese mice induced by high fat diet, in order to discuss the possible mechanisms of PFE in lipid metabolism. Methods: Five-week-old ICR male mice were randomly divided into four groups after adaptive feeding for 1 week: the regular diet(RD) group, the high fat diet(HFD) group, and the HFD + PFE(150, 300mg/kg) group. Medication was given once a day in the HFD+PFE group, while equivalent distilled water was given in the RD group. The body weight was measured twice per week for 4 weeks consecutively. After treatment, mice were sacrificed to measure visceral fat and epididymal fat weight. The plasma concentrations of glucose, insulin, homeostasis model assessment for insulin resistance(HOMA-IR), triglycerides(TG), cholesterol, non-esterified fatty acids(NEFA), aspartate transaminase(AST), and alanine aminotransferase(ALT) were determined. Expression of peroxisome proliferators-activated receptor γ(PPAR-γ) and CAAT-enhancer-binding proteinα(C/EBPα) were respectively detected by Western blot and RT-PCR, and gene expression of SREBP1 and its target gene FAS, SCD1 and GPAT were determined. Results: Compared with the HFD group, PFE reduced the body weight, visceral fat, epididymal fat weight, blood glucose and insulin levels significantly, along with HOMA-IR. Compared with the RD group, expression of PPAR-γ, C/EBPα, SREBP1 and its target gene enhanced in the HFD group, while expression of which were inhibited in the PFE group. Conclusion: PFE could lower body fat and body weight, and regulate lipid metabolism in adipose tissue by suppressing PPAR-γ, C/EBPα, SREBP-1 and related gene expressions in adipose tissue.
出处
《中华中医药杂志》
CAS
CSCD
北大核心
2017年第9期3992-3996,共5页
China Journal of Traditional Chinese Medicine and Pharmacy
基金
国家自然科学基金项目(No.81560605)
吉林省科技厅科技发展计划项目青年科研基金项目(No.20150520148JH)~~
