摘要
目的建立小儿七星茶口服液HPLC指纹图谱,表征小儿七星茶口服液的化学成分信息。方法采用HPLC法建立指纹图谱并用液质联用仪鉴定指纹图谱主要色谱峰。采用Ultimate AQ-C18色谱柱(150 mm×4.6 mm,5μm);以乙腈-0.1%冰醋酸溶液为流动相进行梯度洗脱;检测波长为300 nm;柱温为30℃;体积流量为1.0 m L/min。结果建立了小儿七星茶口服液HPLC指纹图谱,确认并鉴别出共有特征峰12个,21批样品的相似度达0.89以上;方法学考察的精密度、重复性相似度均达1.0,36 h内样品稳定性相似度达0.98。结论所建立的方法稳定、可靠、重复性好,可用于小儿七星茶口服液质量控制。
Objective To establish HPLC fingerprint for Xiaoer Qixing Cha Oral Liquid and analyze its chemical constituents. Methods RP-HPLC method was performed on an Ultimate AQ-C18 （150 mm × 4.6 mm, 5 μm） column with a gradient elution composed of acetonitrile-aqueous solution containing 0,5% acetic acid at the flow rate of 1.0 mL/min. The column temperature was set at 30 ~C, while the detective Wavelength was set at 300 nm. HPLC-MS was used to identify the main chromatographic peaks of the fingerprint. Results The chromatographic fingerprint common pattern was established. Twelve mutual peaks were obtained and identified from the chromatograms of 21 batches of samples and the similarity of the sample was over 0.89. Conclusion The method has good precision, stability, and repeatability and can be used for the quality control ofXiaoer Qixing Cha Oral Liquid.
出处
《中草药》
CAS
CSCD
北大核心
2016年第6期928-932,共5页
Chinese Traditional and Herbal Drugs
基金
2012年度中山市科技计划项目(20123A275)
关键词
小儿七星茶口服液
HPLC
指纹图谱
质量控制
化学成分信息表征
Xiaoer Qixing Cha Oral liquid
HPLC
fingerprint
quality control
chemical constituents information representation
