摘要
目的 :建立山楂药材高效液相色谱指纹图谱。方法 :SymmetryShieldWaters RP1 8柱 ,乙腈 0 5 %醋酸水液梯度洗脱 ,流速 1 0ml·min- 1 ,检测波长 35 5nm ,柱温 4 0℃。结果与讨论 :建立了山楂药材高效液相色谱指纹图谱 。
Object:To establish an HPLC fingerprint of Fructus Crataegus. Method:A symmetryShieldWaters RP 18 column was used,with a mixture solution of acetonitrile and 0 5% acetic acid as mobile phase using a gradient elution.The flow rate was 1ml·min -1 ,the column temperature was 40℃;and the detection wavelength was 355nm.Result and Conclusion:The HPLC fingerprint was established and can be used to identify Fructus Crataegus from different origins.
出处
《中国实验方剂学杂志》
CAS
2004年第4期9-13,共5页
Chinese Journal of Experimental Traditional Medical Formulae
基金
中央级科研院所科技基础性工作 (2 0 0 1DEA2 0 0 1 0 )
中国科学院知识创新工程领域前沿项目 (2F0 2J0 3)
关键词
山楂
HPLC
指纹图谱
Fructus Crataegus
HPLC
Fingerprint
