摘要
目的:建立保和丸(山楂、半夏、陈皮、茯苓、连翘、莱菔子、麦芽、六神曲)水溶性成分高效液相色谱指纹图谱。方法:以0.5的NH4H2PO4溶液为流动相,流速0.8mL/min,检测波长为214nm,绘制保和丸的指纹图谱。结果:建立了保和丸RP-HPLC指纹图谱,精密度和重复性试验中各共有峰相对峰面积的RSD均小于3,符合有关规定。结论:该方法简单,重现性好,具可操作性,可为评价保和丸内在质量的优劣及考察保和丸制备工艺的稳定性提供实验数据。
AIM： To provide a basis for appraising quality standard of Baohe Pill （Fructus Gataegi, Massa medicata fermentata , Rhizoma pinelliae , Pericapium citr reticulatae , Fructus forsythiae , Semen raphani , Fructus Mordei germinatus, Poria） inclusive of its RP-HPLC fingerprint chromatogram. METHODS： The chromatographic column was Kromasil C18 column （4.6 mm ×250 mm, i. d, 5-μm particle size）. The mobile phase was 0.5% solution of ammonium dihydrogen phosphate （ NH4H2PO4 ）, and the flow rate was 0.8 mL/min with UV detector at 214 nm. RESULTS： The RP-HPLC fingerprint chromatogram of Baohe Pill was established. In the experiment, for precision and repeatability, the RSD of each area of common peak was less than 3%. CONCLUSION： This method is simple and reliable.
出处
《中成药》
CAS
CSCD
北大核心
2007年第4期469-473,共5页
Chinese Traditional Patent Medicine
关键词
保和丸
RP—HPLC
指纹图谱
质量
制备工艺稳定性
Baohe Pill
RP-HPLC
fingerprint chromatogram
quality
stability of prepared technology
