摘要
目的:建立假茼蒿药材HPLC指纹图谱,对多批次药材进行质量控制。方法:采用Phenomenex C_(18)ODS2色谱柱(4.6 mm×250 mm,5μm),以乙腈-0.1%磷酸水溶液为流动相梯度洗脱,流速1 m L·min^(-1),检测波长328 nm,柱温30℃;建立假茼蒿药材HPLC指纹图谱,对13批药材进行相似度评价,并进行聚类分析。结果:假茼蒿药材HPLC指纹图谱精密度、重复性和稳定性均较好;13批假茼蒿指纹图谱相似度>0.97;对13批药材进行聚类分析,总共可聚为4类。结论:将HPLC指纹图谱与聚类分析相结合,可对假茼蒿进行质量评价,可为质量控制提供参考。
Objective： To establish the HPLC fingerprint chromatogram of Crassocephalum crepidioides coupled with chemometrics means for the quality control of multi-batches of medicinal material. Method： The separation was developed on C18 column（ Phenomenex,4. 6 mm × 250 mm,5 μm） by gradient elution with acetonitrile-water（ both containing 0. 1% phosphoric acid） as mobile phase at a flow rate of 1. 0 mL·min^-1,the detection wavelength at 328 nm and column temperature at 30 ℃. The HPLC fingerprint chromatogram of C. crepidioides was set up. The quality of 13 batches of medicinal material was evaluated by similarity assay as well as cluster analysis. Result： The establish HPLC fingerprint chromatogram of C. crepidioides was specfic,precise,reproduccible and stable. The similarities of 13 batches of C. crepidioides were more than 0. 97. Further cluster analysis was carried out for the quality consistency of 13 batches of C. crepidioides and accordingly they were devided into 4 clusters. Conclution： With the cluster analysis,the HPLC fingerprint chromatogram C. crepidioides provides a method for qulity control of C. crepidioides.
出处
《中国实验方剂学杂志》
CAS
CSCD
北大核心
2016年第10期76-79,共4页
Chinese Journal of Experimental Traditional Medical Formulae
基金
广西壮瑶药重点实验室项目(桂科基字[2014]32号)
壮瑶药协同创新中心项目(桂教科研[2013]20号)
广西重点学科(壮药学)项目(桂教科研〔2013〕16号)
<广西壮药质量标准(第三卷)>质量评价与标准研究项目(MZY 2013016)
关键词
假茼蒿
高效液相
指纹图谱
聚类分析
Crassocephalum crepidioides
HPLC
fingerprint
cluster analysis
