摘要
目的:明确不同的提取剂对菊芋(Helianthus tuberosus)花盘中次生代谢物质含量和体外抗氧化活性的影响,并探究花盘中的主要的酚类物质。方法:以菊芋花盘干粉为实验材料,使用了不同体积分数的乙醇、甲醇、丙酮等有机溶剂对材料进行提取,测定了总酚、总黄酮含量,并使用FRAP、DPPH、ABTS三种抗氧化方法测定样品的抗氧化活性。通过超效液相色谱的方法测定菊芋花盘中主要的酚类物质。结果:60%乙醇提取的情况下总酚含量最高,70%丙酮提取的情况下总黄酮含量最高,菊芋花盘的抗氧化活性较强并且液相分析结果表明槲皮素是其最主要酚类物质。结论:不同的提取液对菊芋花盘的次生代谢物提取含量影响显著,不同的抗氧化方法均表明菊芋花盘有较强的抗氧化活性,槲皮素作为菊芋花盘的主要酚类物质可以进一步开发利用。
Objective： To study the effect of different extraction agents of Jerusalem artichoke（ Helianthus tuberosus）disk in secondary metabolism substance content and in vitro antioxidant activity.Methods： Using the disk powder of Jerusalem artichoke as raw material,the different volume fraction of ethanol,methanol,acetone and other organic solvents were used to extract the samples,the total phenolic and flavonoids content was determined,and three different types of radical scavenging capacities including the FRAP,DPPH and ABTS were chosen to determine the in vitro antioxidant activity of the samples.By ultra performance liquid chromatography（ UPLC） method,the main phenolics of disk were studied.Results： The highest phenolic content was measured in 60% ethanol extraction.The highest content of total flavonoids was measured in 70% acetone extraction.The Jerusalem artichoke disk showed a strong in vitro antioxidant activity by all three methods and liquid phase analysis results,which showed that the quercerin was the main phenolic in Jerusalem artichoke disk.Conclusion： The different extract had great effect on the secondary metabolics content of Jerusalem artichoke disk and the antioxidant activity of disk was strong.
出处
《食品工业科技》
CAS
CSCD
北大核心
2016年第9期101-105,共5页
Science and Technology of Food Industry
基金
国家自然科学基金项目(31470647)
关键词
菊芋
花
总酚
总黄酮
抗氧化活性
槲皮素
Jerusalem artichoke
disk
total phenolics
total flavonoids
antioxidant activity
quercetin
