摘要
目的：建立夏枯草的融合指纹图谱,实现同分异构体熊果酸和齐墩果酸完全色谱分离和同步含量测定,对夏枯草质量进行评价。方法：采用高效液相色谱法,建立夏枯草的指纹图谱,并为互为同分异构体的熊果酸和齐墩果酸单独建立分析方法,使其达到完全色谱分离,使用＂MATLAB R2012a版＂将两个色谱图融合部分进行基线融合计算,使用＂中药色谱指纹图谱相似度评价系统2004A版＂进行图谱融合。结果：建立了可单独、分段操作的＂模块式＂融合指纹图谱,共标定了18个共有色谱峰,结合质谱和对照品对部分共有峰进行了推断,其中1,3~8,17,18号峰分别推测为丹参素、咖啡酸、芦丁、金丝桃苷、异槲皮苷、异迷迭香酸苷、迷迭香酸、齐墩果酸和熊果酸。同时对15批夏枯草样品中熊果酸和齐墩果酸的含量进行了测定,熊果酸含量0.19%~0.36%,齐墩果酸含量0.055%~0.11%。结论：建立的熊果酸和齐墩果酸的分析方法分离度高、分析速度快、结果准确可靠;建立的＂模块式＂融合指纹图谱可以更准确全面反映夏枯草中的化学成分,可为夏枯草质量控制研究提供参考。
Objective： To establish the fusion fingerprint of prunellae spica, perform the complete chromatographic separation and synchronous content determination of two isomers, ursolic acid and oleanolic acid, and evaluate the quality of prunella spica. Methods ： The fingerprint of prunellae spica was established by HPLC, and a special assay for ursolic acid and oleanolic was established for complete chromatographic separation. The baselines of the fusion part in the two chromatograms were calculated and corrected by ＂MATLAB R2012 a ＂, and the chromatograms were fused by ＂Similarity Evaluation System for Chromatographic Fingerprint of TCM 2004 A ＂. Results ： A ＂modular ＂ fusion fingerprint which could be performed individually and partly was established, in which18 common peaks were included. The peaks 1, 3 ~ 8, 17 and 18 were characterized by MS and standard samples, which were danshensu, caffeic acid, rutinum, hyperoside, isoquercitrin, salviaflaside, rosmarinic acid,oleanolic acid and ursolic acid, respectively. The determination results of ursolic acid and oleanolic acid contents in 15 batches of prunellae spica were 0.19% ~ 0.36% and 0.055% ~ 0.11% respectively. Conclusion ： The developed method for determination of ursolic acid and oleanolic acid showed a high separation resolution, which was rapid, accurate and reliable. The established ＂modular ＂ fusion fingerprint reflected the chemical composition of prunellae spica comprehensively and accurately, which provided a reference for the study on quality control of prunella spica.
出处
《中国执业药师》
CAS
2016年第4期26-32,共7页
China Licensed Pharmacist
基金
深圳市福田区卫生公益性科研项目(FTWS2014028)
山东省中医药科技发展计划(2015-167)
