摘要
目的建立夏枯草HPLC指纹图谱分析方法。方法采用高效液相色谱法,色谱柱为Betasil C18(250mm×4.6mm,5μm)柱,以乙腈-0.1%磷酸梯度洗脱,0～50min时乙腈10%→25%,50～60min时乙腈25%→32%;体积流量为0.7mL/min;检测波长为210nm,柱温25℃。结果建立了夏枯草的指纹图谱,确定了16个共有峰,各夏枯草样品指纹图谱与对照指纹图谱相似度均在0.95以上,可以用于夏枯草药材的定性鉴别。结论此方法简单、准确、重现性好,为夏枯草的质量控制标准提供了有效的方法。
Objective To establish a method of fingerprint analysis on Spica Prunellae by HPLC. Methods The HPLC method was used, chromatographic conditions were Betasil C18 column （250 mm× 4.6 mm, 5 μm）, acetonitrile-0.1% phosphric acid system as mobile phase, 0--50 min acetonitrile 10%→ 25%, 50--60 rain acetonitrile 25%→32% flow rate 0.7 mL/min; detective wavelength at 210 nm, column temperature 25℃. Results Establishing the fingerprint of Spica Prunellae, 16 common peaks were found in the HPLC fingerprint of Spica Prunellae and good similarities with correlation coefficients over 0. 95 were found in the fingerprints between the herbs from different sources and standard fingerprint, which could be utilized for the identification of Spica Prunellae. Conclusion The method is accuate, simple, and could be used for the quality control of Spica Prunellae.
出处
《中草药》
CAS
CSCD
北大核心
2008年第2期272-274,共3页
Chinese Traditional and Herbal Drugs
