摘要
目的基于紫外(UV)和高效液相(HPLC)全息指纹图谱,化学模式识别技术与多成分定量相结合的方法,整合评价不同生长发育时期的49批夏枯草Prunella vulgaris样品化学成分的变化规律,从而阐明夏枯草由草到药的演变过程,解释夏枯草"夏枯质优"的科学内涵。方法建立不同生长发育时期49批夏枯草样品的UV和HPLC全息指纹图谱,采用主成分分析(principal component analysis,PCA)、偏最小二乘法-判别分析(partial leastsquares-discriminant analysis,PLS-DA)对其进行评价,筛选出影响夏枯草分类的特异性吸收波段,并对主要差异性化学成分进行含量测定。结果建立了不同生长发育时期49批夏枯草样品的UV和HPLC指纹图谱,PCA和PLS-DA分析可直观显示夏枯草样品中总的化学组分在不同生长发育时期时动态的变化趋势,其中夏枯草枯萎期可以明显的和其他4个时期区分开来,且其他4个时期有向枯萎期动态渐变的趋势。UV指纹图谱通过PLS-DA筛选出特异性吸收波段为261 nm,HPLC指纹图谱利用PLS-DA的变量因子(VIP)分布图,筛选出VIP值大于1.3的2个色谱峰,经指认为异迷迭香酸苷和迷迭香酸,确定为主要差异性化学成分。对不同生长发育时期的夏枯草差异性化学成分进行含量测定,其中迷迭香酸的含量为0.020%~0.344%;异迷迭香酸苷的含量为0.0016%~0.0988%。多成分定量结果说明,果穗枯萎期中异迷迭香酸苷和迷迭香酸的含量明显增加。含量测定结果与指纹图谱模式识别结果一致,两者可相互验证。结论指纹图谱结合化学模式识别技术与多成分定量,多种分析方法并用,全方位整合的技术手段,实现了对夏枯草从草到药的整个生长发育过程中整体化学信息的动态监测。该方法分析全面、操作简便,为阐明"夏枯质优",规范夏枯草药材采收及全面评价药材质量提供参考。
Objective Based on the UV and HPLC fingerprints of Prunella vulgaris samples, combined with multi-component quantitative analysis and chemical pattern recognition methods, the changes of chemical components in 49 batches of P. vulgaris samples at different growth stages were evaluated, so as to clarify the evolution process of P. vulgaris from herbs to medicines, and explain the scientific connotation of "summer withering with good quality" of P. vulgaris. Methods UV and HPLC holographic fingerprints of 49 batches of P. vulgaris samples at different growth stages were established, which were evaluated by principal component analysis(PCA) and partial least squares-discriminant analysis(PLS-DA). The specific absorption bands affecting the classification of P. vulgaris were screened out. The chemical constituents that cause the difference of P. vulgaris at different growth and development stages were determined. Results UV and HPLC fingerprints of 49 batches of P. vulgaris samples at different growth stages were established. PCA and PLS-DA analysis intuitively showed the dynamic changes of total chemical components in P. vulgaris samples at different growth stages. The withering period of P. vulgaris can be clearly distinguished from the other four periods, and the other four periods had a trend of dynamic gradual change towards the withering period. The specific absorption band of UV fingerprint was 261 nm through PLS-DA. By using the VIP distribution map of PLS-DA, two peaks with VIP value greater than 1.3 were screened out by HPLC fingerprint. Isorosmarin and rosmarinic acid were identified as the main differential chemical components. Determination of chemical components of P. vulgaris in different growth stages was carried out, The content of rosmarinic acid was 0.020%—0.344%. The content of isorosmarin was 0.0016%—0.0988%. Multi-component quantitative results showed that the contents of isorosmarin and rosmarinic acid increased significantly in the withering period of cluster. The results of the content de
作者
刘月新
林艳
谢菁琛
张智敏
廖颖妍
夏伯候
林丽美
LIU Yue-xin;LIN Yan;XIE Jing-chen;ZHANG Zhi-min;LIAO Ying-yan;XIA Bo-hou;LIN Li-mei(School of Pharmacy,Hunan University of Chinese Medicine,Changsha 410208,China;Hunan Key Laboratory for Quality Evaluation of Bulk Herbs,Changsha 410208,China)
出处
《中草药》
CAS
CSCD
北大核心
2021年第7期2082-2090,共9页
Chinese Traditional and Herbal Drugs
基金
长沙市科技计划项目(kq2004063)
湖南中医药大学中药学开放基金项目(2020ZYX11)。
关键词
夏枯草
紫外指纹图谱
高效液相指纹图谱
主成分分析
偏最小二乘法-判别分析
异迷迭香酸苷
迷迭香酸
多成分定量
Prunella vulgaris L.
UV fingerprint
HPLC fingerprint
principal component analysis
partial least squares method-discriminant analysis
isorosmarin
rosmarinic acid
multi-component quantification
