摘要
目的探讨Th17细胞、CD4^+CD25^+Foxp3^+调节性T细胞(Treg)及相关细胞因子IL-6,IL-17,TGF-β1在带状疱疹病程中治疗前后的变化及意义。方法 32例带状疱疹患者分别于治疗前、治疗结束后7d复诊时采外周静脉血,20例健康体检者作为对照组。采用流式细胞分析法检测外周血中Th17,CD4^+CD25^+Foxp3^+Treg细胞占CD4^+T细胞的比例,ELISA法检测外周血IL-6,IL-17,TGF-β1水平。结果带状疱疹患者治疗前、治疗后外周血中Th17,CD4^+CD25^+Foxp3^+Treg细胞的比例明显高于对照组(P均<0.05),治疗前亦明显高于治疗后(P均<0.05),差异均有统计学意义。治疗前、治疗后相关细胞因子IL-17水平显著高于对照组(P均<0.05),治疗前亦显著高于治疗后(P均<0.05),治疗前IL-6,TGF-β1的水平显著高于治疗后及对照组(P均<0.05),差异均有统计学意义;但治疗后与对照组之间差异无统计学意义(P均>0.05)。结论 Th17,Treg细胞在带状疱疹患者外周血中显著增高,在治疗后显著下降,与二者相关的细胞因子也相应变化,提示二者通过多种机制参与机体的抗病毒免疫应答,这在带状疱疹发病机制中可能发挥了重要作用。
Objective To investigate the changes in Th17 and CD4^ ＋CD25^＋ Foxp3^＋ regulatory T（Treg） cells, and their related cytokines （IL-6, IL-17, TGF-β1 ）in patients with herpes zoster after treatment. Methods The venous blood was collected from 32 patients with herpes zoster before and 7 days following completion of treatments and 20 healthy adults. The frequencies of Thl7 cells and Treg cells were detected by flowcytometry, and enzyme-linkedimmuno sorbent assay （ELISA）was used to measure the expression levels of serum IL-6,IL-17, TGF-β1. Results Frequencies of Thl7 and Treg cells, and IL-17 were significantly higher in patients with herpes zoster compared with normal controls（ P 〈 0. 05 ）. Prior to treatment ,the frequencies of Th17 and Treg cells,and IL-17 were significantly higher compared to post treatment （P 〈 0. 05 ）. Serum levels of IL-6, IL-17 ,TGF-β1 were much higher in patients prior to treatment than post-treatment and normal controls （P 〈 0. 05）, while there were no significant differences between post-treatment and normal controls （ P 〉 0. 05 ）. Conclusion Changes in the frequencies Th17 and Treg cells, and the expression levels of their related cytokines are paralleled by disease status, suggesting the potential role of these cells and their cytokines in the pathogenesis of herpes zoster.
出处
《中国皮肤性病学杂志》
CAS
CSCD
北大核心
2016年第5期460-463,470,共5页
The Chinese Journal of Dermatovenereology
