摘要
目的:探讨苦豆碱对氧糖剥夺再灌注损伤后海马神经元的保护作用。方法:以原代培养的新生大鼠海马神经元为研究对象建立氧糖剥夺再灌注损伤模型,MTT法检测神经细胞的存活率,分光光度法检测乳酸脱氢酶(LDH)的漏出率,激光共聚焦显微镜(CLSM)测定海马神经元细胞内Ca2+浓度和线粒体膜电位水平(MMP),利用hoechst33342检测细胞凋亡率。结果:与氧糖剥夺再灌注损伤组比较,苦豆碱(25,50,100 mg/L)组可显著提高细胞存活率,降低乳酸脱氢酶漏出率,抑制Ca2+浓度升高,苦豆碱(25,50,100 mg/L)组能显著降低线粒体膜电位水平,降低细胞凋亡率。结论:苦豆碱对海马神经元氧糖剥夺再灌注损伤具有明显的保护作用。
Objectives： To investigate the protective effects of Aloperine against injury from oxygen-glucose deprivation / reperfusion（ OGD / RP）in primary cultured rat hippocampal neurons. Methods： Cultured hippocampal neurons were exposed to oxygen-glucose deprivation（ OGD）for 2 h followed by a 24 h reperfusion. The MTT assay and the lactate dehydrogenase（ LDH） release were used to evaluate the protective effects of ALO cell viability. Mitochondrial membrane potential（ MMP） and the intracellular free calcium concentration（ [Ca2 ＋]i） in hippocampal neurons were measured using confocal laser scanning microscope（ CLSM）. Hochest 33342 was used to test. Results： Treatment with ALO（ 25 ~ 100 mg / L） significantly attenuated neuronal damage and inhibited LDH release in a dose-dependent manner. Furthermore,inhibited [Ca2 ＋]i elevation and decrease of MMP in ischemia-reperfusion treated hippocampal neurons. Conclusion： These findings suggested that ALO may be a potential neuroprotective agent for cerebral ischemia-reperfusion injury.
出处
《中药药理与临床》
CAS
CSCD
北大核心
2016年第1期44-46,共3页
Pharmacology and Clinics of Chinese Materia Medica
基金
宁夏高等学校科学研究项目(NGY2013073)
关键词
苦豆碱
海马神经元
氧糖剥夺
神经保护
aloperine（苦豆碱）
hippocampal neurons
oxygen-glucose deprivation
reperfusion
neuroprotection
